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Phospho-PNKP (Ser114) Rabbit Polyclonal Antibody#abs140332

Please note that the price mentioned above is for your reference only. For further details regarding the pricing, we kindly request you to get in touch with our seller, Vecent. Western blot analysis PNKP (Phospho-Ser114) using 293 whole cell lysates This product is for research use only, not for...

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Description

Catalog-specification	Delivery time	USD price
abs140332-100ug	1-2 Weeks	301.0
abs140332-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is for your reference only. For further details regarding the pricing, we kindly request you to get in touch with our seller, Vecent.

Overview
catalog	abs140332
Other names	The PNKP gene, also known as polynucleotide kinase 3' phosphatase, encodes for a bifunctional enzyme that possesses both polynucleotide kinase and polynucleotide phosphatase activities. This crucial gene is responsible for repairing DNA damage caused by ionizing radiation and other harmful agents. PNKP mutations have been implicated in a variety of human genetic disorders, including microcephaly with seizures, and zinc deficiency syndrome. In addition to its DNA repair functions, PNKP has also been demonstrated as having potential in cancer therapy, with its role in repairing DNA damage making it a promising target for new anti-cancer drug development. Given the importance of this gene, further research is necessary to fully understand its role in maintaining cellular integrity and to develop potential therapeutic applications.
Source	Rabbit
Specificity	The PNKP (Phospho-Ser114) Antibody specifically recognizes the phosphorylated form of PNKP protein at Serine 114. It effectively detects the levels of endogenous PNKP protein only when it is phosphorylated at this specific site. Please note that this antibody may not be suitable for detecting total PNKP protein levels, as it targets the phosphorylated form specifically at Serine 114.
Species Reactivity	Human
Antigen	PNKP
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	PNKP（114）。，。
MW	60 kDa
Properties
Concentration	1mg/ml
purification	Sequential chromatography on phospho- and non-phospho-peptide affinity columns was used for affinity purification of the antibody from purified rabbit serum. The antibody was obtained through this purification method, which involved the specific binding of the antibody to the phospho-peptide and non-phospho-peptide affinity columns. The purification process resulted in a highly purified antibody with enhanced specificity and affinity.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways. Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either removing 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone.
Tissue specificity	Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine (PubMed:10446192). Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver (PubMed:10446193).
Celluar localization	Mitochondrion;Nucleus;
UniPort	Q96T60