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Phospho-H2AX (Tyr143) Rabbit Polyclonal Antibody#abs140311

Description

Catalog-specification	Delivery time	USD price
abs140311-100ug	1-2 Weeks	301.0
abs140311-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is for reference only. If you require more details on pricing, please get in touch with our sales representative Vecent. It is important to understand that the price quoted may change based on the specific requirements of your order. Therefore, for accurate pricing information, we recommend that you speak to our sales team directly. We will be more than happy to assist you with any queries you may have and provide you with a competitive quote for your needs.

Overview
catalog	abs140311
Other names	H2A histone family member X, also known as H2A.FX or H2A.X, is a key factor in DNA damage response and repair. It is a variant of the H2A histone protein and plays a crucial role in maintaining genome stability. H2AFX, H2AX, H2a/x, Hist5.2ax, Histone 2A, Histone 2AX, Histone H2A.X, Histone H2AX, and RGD1566119 are all alternative names for this protein. Research has shown that H2A.X is rapidly phosphorylated on Ser139 (gamma-H2AX) in response to DNA double-strand breaks induced by ionizing radiation or genotoxic agents. This event amplifies the DNA damage signal and recruits DNA repair factors to the site of damage. H2A.X also plays a critical role in telomere maintenance, replication stress response, and meiotic recombination. H2A.X belongs to a family of highly conserved histone proteins that are involved in compacting the DNA into chromatin and regulating gene expression. However, as a variant of the H2A family, H2A.X has unique features that make it important in DNA damage response. For instance, its C-terminal tail is longer than that of the conventional H2A, giving it the ability to interact with a wide range of proteins involved in DNA repair. In conclusion, H2A histone family member X, or H2A.X, is an essential protein in DNA damage response and repair. Its distinctive features, such as rapid phosphorylation and long C-terminal tail, make it a crucial player in maintaining genome stability.
Source	Rabbit
Specificity	The H2AX (Phospho-Tyr143) Antibody is specifically designed to recognize and bind to the phosphorylated form of endogenous H2AX protein at Tyr143. It is not capable of detecting non-phosphorylated H2AX or H2AX phosphorylated at other sites. Therefore, this antibody can be used as a reliable tool in research related to DNA damage and repair pathways, where H2AX phosphorylation plays a crucial role.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Sheep;Bovine;Dog
Antigen	H2AX
Application	The recommended dilution ratios for WB, IF, and ELISA (peptide) are 1:1000-3000, 1:200, and 1:20000-1:40000, respectively. It's important to note that these ratios may vary depending on the specific experiment and the quality of the sample being used. However, following these guidelines can help ensure optimal results in your research. So, be sure to carefully consider the appropriate dilution ratio for each application in order to obtain accurate and reliable data.
Immunogen	H2AX (143) 。，。
MW	15kDa
Properties
Concentration	1mg/ml
purification	Through a series of sequential chromatography processes using phospho- and non-phospho-peptide affinity columns, the antibody was purified from rabbit serum. The purification method involved affinity purification and resulted in a highly purified antibody derived from rabbit serum. The resulting product is highly similar in structure and function to the original antibody, and is ideal for use in various research applications. Overall, the affinity purification process plays a critical role in the production of high-quality antibodies, and the use of phospho- and non-phospho-peptide affinity columns represents one effective method for achieving this goal.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure the longevity of this product, it is recommended to store it at a temperature of -20°C for a period of one year. It is important to note that exposing the product to repeated freeze/thaw cycles may affect its quality and performance. Therefore, it is advised to avoid such instances and adhere to the recommended storage conditions.
Storage buffer	Store at -20 °C, Rabbit IgG in phosphate buffered saline (pH 7.4) with 150mM NaCl, 0.02% sodium azide, and 50% glycerol. This solution is stable for 12 months from the date of receipt.
Target
Background	Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Tissue specificity	Synthesized in G1 as well as in S-phase.
Posttranslational modification	Phosphorylated on Ser-140 (to form gamma-H2AX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, and may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes. Phosphorylation can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. Widespread phosphorylation may also serve to amplify the damage signal or aid repair of persistent lesions. Phosphorylation of Ser-140 (H2AX139ph) in response to ionizing radiation is mediated by both ATM and PRKDC while defects in DNA replication induce Ser-140 phosphorylation (H2AX139ph) subsequent to activation of ATR and PRKDC. Dephosphorylation of Ser-140 by PP2A is required for DNA DSB repair. In meiosis, Ser-140 phosphorylation (H2AX139ph) may occur at synaptonemal complexes during leptotene as an ATM-dependent response to the formation of programmed DSBs by SPO11. Ser-140 phosphorylation (H2AX139ph) may subsequently occurs at unsynapsed regions of both autosomes and the XY bivalent during zygotene, downstream of ATR and BRCA1 activation. Ser-140 phosphorylation (H2AX139ph) may also be required for transcriptional repression of unsynapsed chromatin and meiotic sex chromosome inactivation (MSCI), whereby the X and Y chromosomes condense in pachytene to form the heterochromatic XY-body. During immunoglobulin class switch recombination in lymphocytes, Ser-140 phosphorylation (H2AX139ph) may occur at sites of DNA-recombination subsequent to activation of the activation-induced cytidine deaminase AICDA. Phosphorylation at Tyr-143 (H2AXY142ph) by BAZ1B/WSTF determines the relative recruitment of either DNA repair or pro-apoptotic factors. Phosphorylation at Tyr-143 (H2AXY142ph) favors the recruitment of APBB1/FE65 and pro-apoptosis factors such as MAPK8/JNK1, triggering apoptosis. In contrast, dephosphorylation of Tyr-143 by EYA proteins (EYA1, EYA2, EYA3 or EYA4) favors the recruitment of MDC1-containing DNA repair complexes to the tail of phosphorylated Ser-140 (H2AX139ph).Monoubiquitination of Lys-120 (H2AXK119ub) by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression (By similarity). Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Ubiquitination at Lys-14 and Lys-16 (H2AK13Ub and H2AK15Ub, respectively) in response to DNA damage is initiated by RNF168 that mediates monoubiquitination at these 2 sites, and 'Lys-63'-linked ubiquitin are then conjugated to monoubiquitin; RNF8 is able to extend 'Lys-63'-linked ubiquitin chains in vitro. H2AK119Ub and ionizing radiation-induced 'Lys-63'-linked ubiquitination (H2AK13Ub and H2AK15Ub) are distinct events.Acetylation at Lys-37 increases in S and G2 phases. This modification has been proposed to play a role in DNA double-strand break repair (By similarity).
Celluar localization	Cytoskeleton;Extracellular region or secreted;Nucleus;
UniPort	P16104