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Phospho-NHE-3 (Ser555) Rabbit Polyclonal Antibody#abs140324

Please note that the price provided is only for your reference. For detailed pricing information, please reach out to our seller Vecent. We suggest contacting Vecent for a precise quote. Western blot analysis NHE-3 (Phospho-Ser555) using EGF treated A431 whole cell lysates This product is for...

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Description

Catalog-specification	Delivery time	USD price
abs140324-100ug	1-2 Weeks	301.0
abs140324-50ug	1-2 Weeks	201.0

Please note that the price provided is only for your reference. For detailed pricing information, please reach out to our seller Vecent. We suggest contacting Vecent for a precise quote.

Overview
catalog	abs140324
Other names	MGC126718 and MGC126720 are genes that encode for the Na(+)/H(+) exchanger 3 protein, also known as NHE3 or sodium/hydrogen exchanger 3. This protein, designated as SL9A3_HUMAN or SLC9A3, plays a crucial role in the transport of sodium and hydrogen ions across cell membranes. Specifically, it functions as a solute carrier, belonging to the SLC9A family, and is a vital component of the apical epithelial sodium/hydrogen exchanger. The SLC9A3 gene, also referred to as Slc9a3, encodes for the isoform 3 of the sodium/hydrogen exchanger within the solute carrier family 9. This family of transporters is responsible for facilitating the exchange of sodium and hydrogen ions between the cell interior and the extracellular environment. In particular, SLC9A3 member 3, or NHE3, ensures the efficient regulation of ion concentrations and pH balance within various tissues and organs. In summary, MGC126718 and MGC126720 are genes that produce the NHE3 protein, a sodium/hydrogen exchanger essential for maintaining ion balance and pH homeostasis in cells. This protein belongs to the SLC9A family and functions primarily at the apical epithelial surface.
Source	Rabbit
Specificity	The NHE-3 (Phospho-Ser555) Antibody is specifically designed to identify the presence of endogenous NHE-3 protein, but only when it is phosphorylated at Ser555.
Species Reactivity	Human;Rat
Predictive reaction species	Chicken;Rabbit;Pig;Dog;Sheep;Horse;Bovine
Antigen	NHE-3
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	Based on the original information, a newly created peptide has been synthesized by incorporating the phosphorylated serine residue at position 555 from human NHE-3 protein.
MW	93 kD
Properties
Concentration	1mg/ml
purification	The purification process of the antibody involves sequential chromatography on phospho- and non-phospho-peptide affinity columns, resulting in the isolation of the antibody from purified rabbit serum.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure the longevity of the product, it is recommended to store it at a temperature of -20 °C for a year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing, as this can compromise its quality and efficacy. Therefore, it is advised to take necessary precautions while storing the product to maintain its potency.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient (PubMed:26358773). Plays an important role in signal transduction.
Posttranslational modification	Phosphorylated by PKA, which inhibits activity. Phosphorylation at Ser-663 by SGK1 is associated with increased abundance at the cell membrane.
Celluar localization	Extracellular region or secreted;Plasma Membrane;
UniPort	P48764