ERBB2 Antibody #abs100477

abs100477-50ul

In Stock

228

Description

Purified Mouse Monoclonal Antibody (Mab)

Other names

The protein receptor tyrosine-protein kinase erbB-2, also known as Metastatic lymph node gene 19 protein (MLN 19) or Proto-oncogene Neu, plays a crucial role in cell growth and differentiation. It is expressed on the surface of some cancer cells, making it a target for certain cancer therapies.
Considered a proto-oncogene, the c-ErbB-2 gene can, when mutated or overexpressed, lead to uncontrolled cell growth. This is the case with HER2-positive breast cancer, where the HER2 gene is overexpressed, and HER2 protein production is upregulated. The resulting overactivity of the ErbB-2 signaling pathway leads to uncontrolled cell proliferation and cancer growth.
Tyrosine kinase-type cell surface receptor HER2, or p185erbB2, CD340, ERBB2, HER2, MLN19, NEU, NGL, are all other names for the ErbB-2 protein. Crucial for normal cell communication, ErbB-2 is also involved in cell adhesion and migration. Understanding the structure, function and regulation of ErbB-2 is critical for developing targeted therapies for HER2-positive breast cancer patients.

Source

Mouse

Specificity

The generation of this ERBB2 antibody was accomplished through the immunization of a mouse with a recombinant protein. Now, I can provide you with a similarly informative statement by reorganizing the original content. Simply put, this antibody was created using a selected protein and by exposing a mouse to it through immunization.

Species Reactivity

Human

Application

WB~~1:1000

Properties

Clonality

Monoclonal Antibody

Clonality number

1423CT594.76.52

Isotype

IgG2b,κ

Stability & Storage

To ensure optimal preservation, it is advised to keep the item refrigerated within the temperature range of 2-8°C for a maximum duration of 14 days. For prolonged storage, dividing the product into small aliquots and maintaining them at a temperature of -20°C is recommended to prevent any potential damage caused by freeze-thaw cycles.

Storage buffer

The monoclonal antibody provided is thoroughly purified and comes in a solution of phosphate-buffered saline (PBS) containing 0.09% (W/V) sodium azide as a preservative. The purification process involves passing the antibody through a protein G column, which effectively isolates and purifies the antibody. Elution from the column is achieved using buffers with high and low pHs, ensuring efficient recovery of the target antibody. To maintain stability and functionality, the eluted antibody is promptly neutralized. Subsequently, the antibody undergoes dialysis against PBS, a process that helps remove any remaining impurities or small molecules. Overall, these purification and preparation methods ensure the delivery of a high-quality monoclonal antibody for various applications.

Research area

Cancer;Signal Transduction

Target

Background

This receptor is a protein tyrosine kinase found in various cell surface receptor complexes that requires a coreceptor for ligand binding. It plays an important role in the neuregulin-receptor complex, although neuregulins alone cannot interact with it. One potential ligand for this receptor is GP30. Its main function is to regulate the growth and stability of peripheral microtubules (MTs). When activated, it initiates the MEMO1-RHOA-DIAPH1 signaling pathway, which leads to the phosphorylation and subsequent inhibition of GSK3B at the cell membrane. This inhibition prevents the phosphorylation of APC and CLASP2, enabling their association with the cell membrane. Consequently, the membrane-bound APC facilitates the localization of MACF1 to the cell membrane, which is crucial for the capture and stabilization of microtubules.

Celluar localization

Isoform 1: Cell membrane; Single-pass type I membrane protein. Cytoplasm, perinuclear region. Nucleus Note=Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1 Isoform 3: Cytoplasm. Nucleus.

UniPort

P04626

References

References

Yamamoto T.,et al.Nature 319:230-234(1986).
Coussens L.,et al.Science 230:1132-1139(1985).
Wakamatsu A.,et al.Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases.
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Tal M.,et al.Mol. Cell. Biol. 7:2597-2601(1987).