CSF1R Antibody #abs100498

abs100498-50ul

In Stock

228

Description

Purified Mouse Monoclonal Antibody (Mab)

Other names

The CSF-1 receptor, also known as macrophage colony-stimulating factor 1 receptor, M-CSF-R, Proto-oncogene c-Fms, CD115, CSF1R, or FMS, is an essential component of the immune system. It plays a crucial role in the regulation of macrophage proliferation, differentiation, and survival. It is a transmembrane receptor that binds to the cytokine colony-stimulating factor 1 (CSF-1) and mediates its biological effects. CSF-1R is expressed on the surface of monocytes, macrophages, and their progenitors. Dysregulation of CSF-1R signaling has been implicated in several diseases, including cancer and inflammatory disorders. Targeting CSF-1R has emerged as a potential therapeutic strategy for these diseases.

Source

Mouse

Specificity

The generation of this CSF1R antibody involves immunizing a mouse with a recombinant protein. Let's create a highly similar content by rearranging the provided information. Please note that the generated content is derived from the original text.
"Through immunization with a recombinant protein, this CSF1R antibody is developed. The procedure includes the use of a mouse to generate the desired immune response. By exposing the mouse to the recombinant protein, its immune system is activated, leading to the production of specific antibodies targeting the CSF1R protein."

Species Reactivity

Human

Application

IHC-P~~1:25
FC~~1:25
WB~~1:4000

Properties

Clonality

Monoclonal Antibody

Clonality number

1486CT328.53.37

Isotype

IgG1,k

Stability & Storage

To keep the product fresh and effective, it is important to store it at a temperature between 2 and 8°C for up to 2 weeks. For longer term storage, it is recommended to keep it in smaller aliquots and store them at -20°C to prevent the freeze-thaw cycles. This will ensure that the product remains stable and usable for an extended period of time. So, always make sure to store the product as per the given instructions to reap maximum benefits.

Research area

Stem Cells

Target

Background

CSF1R, also known as colony-stimulating factor 1 receptor, is a tyrosine-protein kinase that acts as a cell-surface receptor. It plays a crucial role in the regulation of survival, proliferation, and differentiation of hematopoietic precursor cells, particularly mononuclear phagocytes like macrophages and monocytes. Additionally, it is involved in innate immunity, inflammatory processes, and the release of proinflammatory chemokines in response to IL34 and CSF1.
CSF1R is essential for normal bone and tooth development, as well as the regulation of osteoclast proliferation and differentiation. It also plays a significant role in male and female fertility, and the development of milk ducts and acinar structures in the mammary gland during pregnancy. Moreover, it promotes the reorganization of the actin cytoskeleton, regulates membrane ruffles, cell adhesion, and cell migration. In cancer cells, it promotes invasion.
Upon ligand binding, CSF1R activates several signaling pathways. It phosphorylates various proteins including PIK3R1, PLCG2, GRB2, SLA2, and CBL. Phosphorylation of these proteins leads to the activation of downstream signaling molecules and pathways, such as the AKT1, MAPK, and SRC family kinase pathways. CSF1R also activates STAT family members (STAT3, STAT5A, and STAT5B) and promotes the tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1.
However, CSF1R signaling is down-regulated by protein phosphatases like INPP5D/SHIP-1, which dephosphorylate the receptor and its downstream effectors. Additionally, the activated receptor is rapidly internalized, further contributing to the termination of receptor signaling.

Celluar localization

Cell membrane; Single-pass type I membrane protein

UniPort

P07333

References

References

Hampe A.,et al.Oncogene Res. 4:9-17(1989).
Coussens L.,et al.Nature 320:277-280(1986).
Andre C.,et al.Genomics 39:216-226(1997).
Jin P.,et al.Arthritis Res. Ther. 10:R73-R73(2008).
Schmutz J.,et al.Nature 431:268-274(2004).