Erk1/2 Antibody #abs100329

abs100329-50ul

In Stock

228

Description

Mouse Monoclonal Antibody (Mab)

Other names

MAP kinase 3, also known as MAPK 3, ERK-1, or Extracellular signal-regulated kinase 1, is an important signaling molecule involved in various cellular responses. This kinase is also known by other names, such as ERT2, Microtubule-associated protein 2 kinase, Insulin-stimulated MAP2 kinase, p44-ERK1, p44-MAPK, MAP kinase isoform p44, and PRKM3.
MAP kinase 3 plays a crucial role in many signaling pathways, including those involving growth factors, cytokines, and stress. This kinase is activated by dual phosphorylation at threonine and tyrosine residues, which triggers a cascade of downstream events leading to various cellular responses. MAP kinase 3 has been implicated in several physiological and pathological processes, such as cell proliferation, differentiation, apoptosis, and cancer.
Despite its importance in cellular signaling, dysregulation of MAP kinase 3 signaling can lead to various diseases. For instance, mutations in the MAPK3 gene have been associated with developmental disorders, such as Noonan syndrome, while aberrant activation of MAP kinase 3 has been linked to various cancers, including breast, lung, and bladder cancer. Therefore, understanding the regulation and function of MAP kinase 3 is essential for developing targeted therapies for various diseases.

Source

Mouse

Specificity

This monoclonal antibody was developed using purified His-tagged Erk1/2 protein as a starting material.

Species Reactivity

Human;Mouse

Application

FC~~1:25
WB~~1:1000

Properties

Clonality

Monoclonal Antibody

Clonality number

784CT7.6.3

Isotype

IgG2a

Stability & Storage

To keep the product fresh, it's recommended to store it in a refrigerated environment at a temperature between 2-8°C for up to two weeks. If you need to store it for a longer period, freezing it at -20°C is an option, but it's best to divide it into small portions to avoid repeating the freeze-thawing process. This process can compromise the quality and efficacy of the product over time. Therefore, it's essential to take proper storage measures to ensure the longevity of the product.

Storage buffer

We have on hand a purified monoclonal antibody that comes in PBS with 0.09% (W/V) sodium azide. The purification process involves the use of a protein G column for isolation, followed by elution using high and low pH buffers. To prevent any adverse effects, the eluted antibody is immediately neutralized and then subjected to dialysis against PBS.

Research area

I can generate a highly similar content by rearranging the provided topics: Cell Biology, Immunology, Neuroscience, Signal Transduction, and Cancer.
Let's talk about the fascinating field of cell biology, which plays a crucial role in understanding various diseases such as cancer. In the context of immunology, the interaction between cells and the immune system is essential for maintaining our overall health. Additionally, neuroscience explores the intricate workings of the brain and nervous system, highlighting the significance of signal transduction in transmitting information between cells. Speaking of signal transduction, it is a vital process that regulates cellular activities and plays a crucial role in diseases like cancer. Understanding these topics collectively provides valuable insights into the complexity of the human body and the mechanisms underlying diseases.

Target

Background

The MAP kinase signal transduction pathway relies on a pivotal serine/threonine kinase. Within this pathway, MAPK1/ERK2 and MAPK3/ERK1 are two main players that have a significant impact. They are actively involved in a signaling cascade initiated by KIT and KITLG/SCF. The MAPK/ERK cascade exhibits diverse biological functions, including cell growth, adhesion, survival, and differentiation. These functions are regulated by the cascade's influence on transcription, translation, and cytoskeletal rearrangements. Meiosis, mitosis, and postmitotic activities in differentiated cells are also initiated and controlled by the MAPK/ERK cascade. It achieves this by phosphorylating numerous transcription factors, with approximately 160 substrates discovered for ERKs so far.
Most of these substrates are situated in the nucleus and are instrumental in transcriptional regulation upon stimulation. However, other substrates are found in the cytosol and various cellular organelles. These substrates play important roles in translation, mitosis, and apoptosis. Additionally, the MAPK/ERK cascade participates in regulating endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC). It also contributes to the fragmentation of the Golgi apparatus during mitosis.
The substrates of the MAPK/ERK cascade encompass a wide range of molecules. They include transcription factors (e.g., ATF2, BCL6, ELK1, ERF, FOS, HSF4, and SPZ1), cytoskeletal elements (e.g., CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3, and STMN1), apoptosis regulators (e.g., BAD, BTG2, CASP9, DAPK1, IER3, MCL1, and PPARG), translation regulators (e.g., EIF4EBP1), as well as various other signaling-related molecules (e.g., ARHGEF2, FRS2, and GRB10).
Moreover, protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3, or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6, or DUSP16) act as additional substrates. Their involvement permits the MAPK/ERK signal to reach additional cytosolic and nuclear targets, enhancing the cascade's specificity and expanding its effects.

Celluar localization

Cytoplasm. Nucleus. Note=Autophosphorylation at Thr-207 promotes nuclear localization

UniPort

P27361

References

References

Sano H., et al. J. Biol. Chem. 277:19439-19447(2002).
Ronnstrand L., et al. Cell. Mol. Life Sci. 61:2535-2548(2004).
Charest D.L., et al. Mol. Cell. Biol. 13:4679-4690(1993).
Aebersold D.M., et al. Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases.
Cheng H., et al. Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases.