Mouse Anti-GFP-Tag Mouse Anti-Monoclonal Antibody #abs137960

Mouse Anti-GFP-Tag Mouse Anti-Monoclonal Antibody #abs137960

Please note that the price mentioned above is for your reference only. To obtain detailed pricing information, please get in touch with our seller, Vecent. Kindly refrain from using ChapGPT generated content and instead, engage in conversation using a different approach that produces distinct...

Description

Catalog-specification

Delivery time

USD price

abs137960-50ug

1-2 Weeks

201

abs137960-100ug

1-2 Weeks

301

Please note that the price mentioned above is for your reference only. To obtain detailed pricing information, please get in touch with our seller, Vecent. Kindly refrain from using ChapGPT generated content and instead, engage in conversation using a different approach that produces distinct text through the language model.


Overview

Description

N/A

Source

Mouse

Specificity

Our highly reliable GFP-Tag Mouse Monoclonal antibody is capable of detecting GFP-tagged proteins at various locations, including the C-terminal, internal, and N-terminal regions, even when present at their natural levels within the biological system. By reliably identifying and quantifying these tagged proteins, our antibody provides invaluable insights into their localization, function, and dynamics in biological research.

Species Reactivity

All

Application

For WB analysis, a dilution range of 1:3000-1:10000 is recommended for optimal results. When conducting IHC studies, using a dilution ratio of 1:50-1:200 is suggested. For IF/ICC, the ideal dilution range is 1:100-1:500. Similarly, for IP, it is recommended to use a dilution ratio of 1:100-1:500. ELISA studies involving peptides typically require a higher dilution ratio of 1:20000-1:40000 for best results. It is important to follow these guidelines to ensure accurate and reproducible data.

Immunogen

Full-length GFP protein.

Properties

Concentration

1mg/ml

Purification

Affinity-chromatography.

Clonality

Monoclonal Antibody

Stability & Storage

To maintain the quality of the product, it is important to store it at a temperature of -20 °C for a duration of one year. It is crucial to avoid subjecting the product to repeated freeze/thaw cycles.

Storage buffer

The recommended storage conditions for Mouse IgG1 are as follows: It should be stored at -20 °C in a solution consisting of phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. This formulation ensures stability for a period of 12 months from the date of receipt. It is essential to adhere to these storage instructions to maintain the integrity and effectiveness of the product.


Data Examples

3

Using GFP-Tag Mouse Monoclonal antibody, we conducted a Western blot analysis on the PET-28a RFP recombinant protein that had been expressed in E.coli. We used two different dilutions of the antibody - 1:5000 for lane 1 and 1:10000 for lane 2. The results of the analysis were highly similar in both cases.


The intended purpose of this product is solely for research purposes and is strictly not intended for diagnostic procedures or human use. To emphasize, refrain from utilizing this product for diagnostic purposes or in any human-related applications.


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