Humanized Anti-human CD3 Mab #abs160014

abs160014-500ug

In Stock

328

Description

T cells rely heavily on the CD3 molecule, an essential symbol within their ranks. This molecule comprises five polypeptide chains, namely γ, δ, ε, ζ, and η. These chains possess cytoplasmic regions housing ITAM, instrumental in transmitting signals from the T cell receptor (TCR) and activating T cells. Through non-covalent bonding, the CD3 molecule and TCR form the formidable TCR-CD3 complex, tasked with relaying activation signals from TCR and antigens to the cell, consequently initiating T cell activation. To harness the tumor cell-killing potential of mononuclear cells derived from peripheral blood, bone marrow, or umbilical cord blood, researchers subject them to cultivation in the presence of CD3 monoclonal antibody and multiple cytokines for a specified duration. Successfully cultured, these cells transform into CIK (Cytokine-induced Killer) cells, proficient in targeting and eliminating tumor cells.

Endotoxin (Endotoxin) <2EU/mg

Other names

Anti-human CD3 monoclonal antibody

Source

CHO cells

Instructions

We suggest utilizing a concentration of 400ng/ml for the soluble method. This concentration effectively triggers complete cell activation and stimulation, resulting in notable enhancements in the cell expansion factor and the final CD3+CD56+ Shuangyang rate. It is crucial to maintain this recommended concentration for optimal results.

The T75 square flask was coated using the following method: 10mL of PBS buffer containing 5ug/mL of anti-human CD3 was spread evenly on the flask's surface. The flask was then placed in a refrigerator at 4℃ and left overnight for the coating to occur. Before using the flask, it is important to remove the PBS buffer and wash the flask three times with saline solution. This process ensures the removal of any excess buffer and prepares the flask for further use.

Properties

Form

Colorless transparent liquid

Concentration

1mg/mL

Purification

ProteinA affinity chromatography

Clonality number

OKT3

Stability & Storage

To ensure the quality of the product, it is best to store it in a temperature-controlled environment between 2 and 8℃. The shelf life is 2 years. After dissolving and packaging the liquid, it is recommended to store it at -20℃ for up to 6 months or -80℃ for up to 12 months to prevent repeated freezing and thawing. This will help maintain the product's stability and efficacy over time.