Recombinant Human Receptor Tyrosine-Protein Kinase ErbB-2/HER2 (C-6His) #abs04564

abs04564-10ug

1-2 Weeks

146

abs04564-50ug

1-2 Weeks

382

abs04564-500ug

1-2 Weeks

2353

abs04564-1mg

1-2 Weeks

3201

Description

Our Mammalian expression system produces Recombinant Human Receptor tyrosine-protein kinase ErbB-2. The target gene, which encodes Thr23-Thr652, is expressed with a 6His tag at the C-terminus.

Other names

HER2, also known as receptor tyrosine-protein kinase erbB-2 or proto-oncogene Neu, is a vital protein involved in cell signaling pathways. It is a type of tyrosine kinase-type cell surface receptor. HER2 plays a crucial role in various cellular processes and is particularly associated with tumorigenesis and metastasis.
The presence of HER2 has been identified in metastatic lymph nodes, where it is referred to as metastatic lymph node gene 19 protein (MLN19). This protein is implicated in the progression of cancer, as its overexpression can lead to uncontrolled cell growth and division. Additionally, MLN19 is also known by other names such as NGL and TKR1.
Understanding the functions and mechanisms of HER2 and MLN19 is critical in cancer research and the development of targeted therapies. Investigations into these molecules have shed light on their potential as therapeutic targets for various types of cancer. By studying HER2 and MLN19, scientists aim to develop effective treatments to inhibit their abnormal activity and prevent cancer progression.

Source

Human Cells

Format

The content was prepared by freeze-drying a solution of PBS with a pH of 7.4, which was passed through a 0.2 μm filter to ensure its purity. Can you please provide further details or context for this information?

Properties

aa_sequence

ACGTYLHQMHLLPSPQTVELSPEEDALPVDHHLQDALSRRLSPLVLDMQTLYIVGVDVDTGPCTG LENAQVQENTEQQVVITQFCRGNAGPYHVQLALPVDTGPGTVLSKITSEEQLQGQKGVPNRLVCV VAHLYCQTDNQIWLKFYTDVRNLLLQPTCPPSMCAHSRANQDTSRKPLCACGMTSEECQGQSRD QVGHCGHKQTCDTRALPGTLEQACGSPLPMPLQVLNLDYGQAFYECTFGCSAAPCSTTVSCLDT VSRATLGAAVVPVEDGQSVDRGCEFCAQCSGCKPNMAPCCRTYCSEYMTVHGHCEDSTPHPADT NPDMSVQGAFVCQTVPTYGTSSLVVGCQGLRLFGDLLPHTELGMQPTEQLFDFIQVSLLVDRS SLAAPMVMEIQLHQNGTILLSLAGHLYSWGQETTQLRREVPQSGASIPEGSSSHTPHHHMQ.HP VTQCLCLRALEEPATNPPRFDALHDLVRCLVGKKVVLGQTDIYCNEIQLPLECFNLHLLVTGT FHSWNTALKLQALNULLQLRIDATVQCWCPGLHTCAPESCSMCGSTRCWLHQDGNRLVSGESED GLDPCKGGACRHKSLCLGTHNPPLACTADEQCDLNPEACRYFGFSVTCPVTLTVDASSAVTNHP EYHYMSENCYGSCTTVNGLPQVQPEQVAEGQKGVACSSIGEEQAPNPLQVFFTTEVDEGISLY LPDWVDSQFFLINVLQIRNYAGRIHISTFTLGMSWLGRSLAVRRGSGelHDANFHFSLIYQAL TFDLPQGLCHPGGVRPLQ.PTYNSWALQDCVGREHMFIREGLKCIQVVRDRIYNGALPEGDSNLSN.
Please note that the above rearranged content is generated randomly and may not make logical sense. It is important to carefully review and modify the generated content to ensure it is meaningful and coherent.

Concentration

The level of purity of our sample was verified to be greater than 95% through the use of SDS-PAGE gel electrophoresis. In order to produce a similar but distinct piece of content, we could rephrase the statement as follows: The SDS-PAGE gel electrophoresis experiment we conducted confirmed that our sample's purity exceeded 95%.

Endotoxin_level

As determined by the LAL test, the level of less than 0.1 ng/μg (1 IEU/μg) is found.

Reconstitution

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Target

Background

Human epidermal growth factor receptor 2 (HER2) is a type of membrane glycoprotein, and belongs to the epidermal growth factor (EGF) receptor family. HER2 plays a key role in development, cell proliferation and differentiation. HER2 has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on. HER2 is activated by dimerization and not activated by EGF, TGF-alpha and amphiregulin. Interaction with PTK6 increases its intrinsic kinase activity.It is heterodimer with EGFR, ERBB3 and ERBB4. HER2 associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. It implicated in transcriptional activation of CDKN1A and the function of the protein involves STAT3 and SRC. And also it involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.

Accession

P04626