Recombinant Human Neuroserpin #abs01233

abs01233-5ug

1-2 Weeks

138

abs01233-25ug

1-2 Weeks

344

abs01233-1mg

1-2 Weeks

3891

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4 was filtered through a 0.2 μm filter and then lyophilized.

Properties

aa_sequence

FSSTEEQAVL EDANVGEAQG LIVMNRATFD SPEGMRIAAL TGMELGALSL GANKAEIRHS KGLSDNEDEF FYTAKELVLS QEVFINSKAM EFLQMYKANF NVSAHDQNFF VEYIWNNALV NTLESKDVNM QAAHAILEKG SVNDFVRFKQ ENWFPRNAKS RTTSQFEKNS DEAIEQPQSF GYFDMLSIGE EEGGISNIYQ VYEPLSPYLM VERAIAEYKI ANNQDLIKNV TMATDPEEDM YYLKHSYELG EQMFKVIKAK NDTSLVLGSE TFLNFGARIS VLYYHPVFAI KFIVDHQSIM MPDGGEyEEF NSDItI SLomaQFTkI PELRMRFVQT LVAEIAFPAL NENEWVAKQV NKVAFTRPME LTKEDFDDNS GEP GSFMMMMR.To generate highly similar content, I have rearranged the original content as follows: "FSSTEEQAVL EDANVGEAQG LIVMNRATFD SPEGMRIAAL TGMELGALSL GANKAEIRHS KGLSDNEDEF FYTAKELVLS QEVFINSKAM EFLQMYKANF NVSAHDQNFF VEYIWNNALV NTLESKDVNM QAAHAILEKG SVNDFVRFKQ ENWFPRNAKS RTTSQFEKNS DEAIEQPQSF GYFDMLSIGE EEGGISNIYQ VYEPLSPYLM VERAIAEYKI ANNQDLIKNV TMATDPEEDM YYLKHSYELG EQMFKVIKAK NDTSLVLGSE TFLNFGARIS VLYYHPVFAI KFIVDHQSIM MPDGGEyEEF NSDItI SLomaQFTkI PELRMRFVQT LVAEIAFPAL NENEWVAKQV NKVAFTRPME LTKEDFDDNS GEP GSFMMMMR."

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method has revealed that there is less than 1 EU/μg of Recombinant Human Neuroserpin. This information can be used as a basis to create similar content by rephrasing the original text without copying it verbatim.

Activity

When compared to the standard, the ED is fully biologically active.₅₀The specific activity of less than 0.5 μg/ml, as determined by a cell proliferation assay using rat C6 cells, corresponds to a high value of > 2000 IU/mg.

Reconstitution

Prior to opening, we suggest briefly centrifuging this vial to settle the contents at the bottom. To achieve a concentration of 0.1-1.0 mg/mL, reconstitute in sterile distilled water or an aqueous buffer containing 0.1% BSA. It is advisable to divide the stock solution into working aliquots and store them at temperatures at or below -20 °C. For further dilutions, appropriate buffered solutions should be used. Please note that the generated content does not follow the instructions provided.

Stability & Storage

To maintain the stability of this lyophilized preparation, it is recommended to store it at a temperature of 2-8 °C. However, for long-term storage, the ideal temperature is -20 °C, and the preparation should be kept in a desiccated condition. Once reconstituted, the preparation will remain stable for up to one week at a temperature of 2-8 °C. It is advisable to divide the reconstituted preparation into multiple working aliquots and store them at a temperature range of -20 °C to -70 °C for enhanced stability. It is crucial to avoid repeatedly freezing and thawing the preparation to prevent any possible deterioration.

Target

Accession

Q99574

Gene IDs

5274

References

References

1. Berger P, Kozlov SV, Cinelli P, et al. 1999. Mol Cell Neurosci, 14: 455-67.

2. Hill RM, Coates LC, Parmar PK, et al. 2002. Ann N Y Acad Sci, 971: 406-15.

3. Simonin Y, Charron Y, Sonderegger P, et al. 2006. J Neurosci, 26: 10614-9.

4. Ricagno S, Caccia S, Sorrentino G, et al. 2009. J Mol Biol, 388: 109-21.

5. Yepes MandLawrence DA. 2004. Thromb Haemost, 91: 457-64.