Recombinant Human MIC-B #abs01259

abs01259-10ug

1-2 Weeks

138

abs01259-50ug

1-2 Weeks

344

abs01259-1mg

1-2 Weeks

3079

Species

Human

Source

Escherichia coli.

Format

A concentrated solution in 20 mM Tris, 150 mM NaCl, pH 8.0 was filtered through a 0.2 μm filter and then lyophilized.

Properties

aa_sequence

DEQSTVPLQS ALAMNTFWKE GRYLKSGVIA RTVPPLVNVT CSEVSEGTIN VXCRASSFYP RNITLTWDRQ GVSLSHNTQQ WGDVLPDGNG TYQTWVATRI RQGEEQRFTC YMEHSGNHGT HPVPSGKVLV LQSQRTDQEG THPHRQMTQV GFLAEGHDLQ KRRAGPQWAE DLGAKTWDTV EDLTNENQD QLRTLTHGI DQKGGLHSK LQEIRVCEI HDSSTRGSR FYDQETLNQS STPQASQLMN VNFWKEDAMK TKHYRAMQA DCLQKLQRY MVLSYRSNEP FLRYDRQKA.

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

As determined by the LAL method, the Recombinant HumanMIC-B has a level of less than 1 EU/μg. To create a similar statement, the information provided should be rephrased and presented in a different manner without changing its original meaning.

Activity

The standard and the fully biologically active variant were compared, and their specific activity was assessed by measuring their ability to bind MICB antibody in an ELISA assay. To generate similar content, the rearranged version could be as follows:
The specific activity of the fully biologically active variant was determined by examining its binding capacity to MICB antibody in an ELISA assay, and it was found to show equivalency to the standard.

Reconstitution

To ensure optimal performance, it is suggested that the vial containing the sample be centrifuged briefly before opening to settle the contents at the bottom. Reconstitution should be carried out using sterile, distilled water or an aqueous buffer, preferably containing 0.1% BSA, to achieve a concentration of 0.1-1.0 mg/ml. Stock solutions should be divided into working aliquots and stored at a temperature not exceeding -20 °C. For further dilutions, appropriate buffered solutions should be used.

Stability & Storage

For optimal storage conditions of this lyophilized preparation, it is recommended to keep it at a temperature range of 2-8 °C. However, for long term storage, it is advisable to store it at -20 °C, preferably in a desiccated state. Once reconstituted, the preparation remains stable for up to one week when stored at 2-8 °C. To ensure maximum stability, it is suggested to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles as it may affect its stability.

References

References

1. Komatsu-Wakui M, Tokunaga K, Ishikawa Y, et al. 1999. Immunogenetics, 49: 620-8.

2. Holmes MA, Li P, Petersdorf EW, et al. 2002. J Immunol, 169: 1395-400.

3. Gambelunghe G, Falorni A, Ghaderi M, et al. 1999. J Clin Endocrinol Metab, 84: 3701-7.