Recombinant Human GDNF #abs00928

abs00928-2ug

1-2 Weeks

138

abs00928-10ug

1-2 Weeks

344

abs00928-1mg

1-2 Weeks

6742

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in 1 × PBS, pH 7.4, with 0.05% Tween-20 was filtered through a 0.2 μm filter and then lyophilized.

Properties

aa_sequence

LAVPSPDKQM ARRERNRQAA AKGSRGKNEP NRGQRGKCVR LNVTAIHRLT EKYGLLYTDE FRYCSGEELI DAETSCDNAA RNRKLSNLKI DVGQASDVLW FDDDIAPCRP VYDNLLDFS HLIRKHIAKS RCGC，，。

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The Recombinant Human GDNF has been determined to have a very low endotoxin level of less than 0.1 EU/μg using the LAL method.

Activity

，。 ED。，。₅₀The specific activity of the product, as evaluated by a cell proliferation assay with rat C6 cells, proves to be greater than 5.0 x 10, while the minimal effective concentration is less than 0.1 ng/ml. These findings validate the potency of the product to elicit a significant biological response.⁷ IU/mg.

Reconstitution

Prior to opening, it is recommended to briefly centrifuge this vial in order to settle the contents at the bottom. The reconstitution should be done using sterile distilled water or an aqueous buffer that contains 0.1% BSA, aiming for a concentration of 0.1-1.0 mg/mL. To ensure stability, stock solutions should be divided into working aliquots and stored at or below -20 °C. When making further dilutions, use appropriate buffered solutions.

Stability & Storage

The lyophilized preparation can be stored at 2-8 °C, but for long-term storage it is best to keep it at -20 °C while ensuring that it is desiccated. When reconstituted, the preparation remains stable for up to one week at a temperature range of 2-8 °C. To achieve maximum stability, it is recommended to divide the reconstituted preparation into working aliquots and store them at a temperature range of -20 °C to -70 °C. Repeated freeze/thaw cycles should be avoided. These measures are important to maintain the integrity and efficacy of the preparation over time.

Target

Accession

P39905

Gene IDs

2668

References

References

1. Wood MD, Kim H, Bilbily A, et al. 2012. Muscle Nerve, 46: 122-4.

2. Stahl K, Mylonakou MN, Skare O, et al. 2011. Brain Res, 1378: 105-18.

3. Cornejo M, Nambi D, Walheim C, et al. 2010. Neurochem Res, 35: 1643-51.

4. Boado RJ, Zhang Y, Wang Y, et al. 2008. Biotechnol Bioeng, 100: 387-96.