Recombinant Human MANF #abs00929

abs00929-5ug

1-2 Weeks

138

abs00929-25ug

1-2 Weeks

344

abs00929-1mg

1-2 Weeks

3891

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4 was filtered through a 0.2 μm filter and subsequently lyophilized. Now, please generate alternative content using a different approach that results in a significantly divergent text from the original information.

Properties

aa_sequence

Dear friends and colleagues,
I hope this message finds you well. I wanted to take a moment to share some important information with you. It has come to my attention that there are certain developments that require our immediate attention.
In recent times, the organization has been facing several challenges. We must stay strong and united to overcome these obstacles. Our team has always been known for its resilience and determination, and I firmly believe that we have what it takes to overcome any hurdles that come our way.
Teamwork and effective communication are crucial in times like these. We need to come together and brainstorm innovative solutions to the problems we are currently facing. Each and every one of us plays a vital role in this process, and it is only by working collaboratively that we can achieve success.
I am confident in the abilities of each team member and have seen firsthand the incredible talent and dedication that you all possess. By leveraging these strengths and supporting each other, I am positive that we can navigate through these challenging times and come out stronger than ever.
It is essential that we maintain a positive mindset and stay focused on our goals. Let us not get discouraged by temporary setbacks but rather view them as opportunities for growth and improvement. Together, we can turn these challenges into stepping stones towards future success.
Please remember to take care of yourselves and each other. Your well-being is of utmost importance, both personally and professionally. Reach out to your teammates if you need support or guidance. We are here for each other, and together, we can grow and thrive.
Thank you for your unwavering commitment and hard work. I am privileged to be a part of such an exceptional team, and I know that together, we will accomplish great things.
Best regards,
[Your Name]

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The concentration of Recombinant Human MANF is determined to be below 1 EU/μg using the LAL method. Let me know if there is anything specific you would like to know about this information.

Activity

The standard and the ED are compared in terms of their biological activity, and it is found that the ED is fully biologically active.₅₀The specific activity of a substance, as determined through a cell proliferation assay utilizing rat C6 cells, is above 50 IU/mg when the concentration is less than 20 μg/ml. This information indicates the potent nature of the substance and its ability to stimulate cell growth and division. These findings provide valuable insights into the potential uses of the substance in fields such as medicine and biotechnology. It is important to note that further research and experimentation are necessary to fully understand the physiological effects of this substance.

Reconstitution

To ensure proper mixing, it is recommended to briefly centrifuge the vial before opening to settle the contents at the bottom. Prepare a solution by reconstituting in either sterile distilled water or an aqueous buffer containing 0.1% BSA, aiming for a concentration between 0.1-1.0 mg/mL. For long-term storage, divide the stock solution into smaller working aliquots and keep them frozen at temperatures below or equal to -20 °C. When creating further dilutions, use suitable buffered solutions.

Stability & Storage

To ensure the stability of this lyophilized preparation, it should be stored at a temperature range of 2-8 °C, although for long term storage, it is advisable to keep it at -20 °C, making sure it is desiccated. After reconstitution, the preparation can remain stable for up to a week at 2-8 °C. For optimal stability, it is recommended to divide the reconstituted preparation into working aliquots and keep them at a temperature range of -20 °C to -70 °C. It is essential to avoid repeatedly undergoing freeze/thaw cycles to maintain the stability of the preparation.

Target

Accession

P55145

Gene IDs

7873

References

References

1. Petrova P, Raibekas A, Pevsner J, et al. 2003. J Mol Neurosci. 20:173-88.

2. Airavaara M, Chiocco MJ, Howard DB, et al. 2010. Exp Neurol. 225:104-13.

3. Lindholm P, Peranen J, Andressoo JO, et al. 2008. Mol Cell Neurosci. 39:356-71.