Recombinant Human Epigen #abs00901

abs00901-5ug

1-2 Weeks

138

abs00901-25ug

1-2 Weeks

344

abs00901-1mg

1-2 Weeks

3891

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4 was filtered through a 0.2 μm filter and then lyophilized.

Properties

aa_sequence

LCEYAQPITV TPNQDEAGI QLKFSCHCLE GSYDNHICNA HACEFLEKHL CKIGCTYFTR TELHEHCSTR G.

Concentration

> 98 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined the presence of less than 1 EU/μg of Recombinant HumanEpigen. Let me generate a revised statement using the same information but in a different structure: The concentration of Recombinant HumanEpigen was determined using the LAL method, revealing a minimal presence of fewer than 1 EU/μg.

Activity

The biological activity of the sample was found to be fully comparable to that of the standard product. Both exhibited the same level of ED or effective dose, indicating the similarity in their activity.₅₀The specific activity of a protein can be measured by using a cell proliferation assay with Balb/c 3T3 cells. In the case of this particular protein, the concentration required to achieve a response of less than 50% cell proliferation is less than 300 ng/ml. This indicates a high specific activity, greater than 3.3 × 10 as measured by the assay. It is important to note that this information is based on the original text and was generated using a language model approach that resulted in unique phrasing and sentence structure.³ IU/mg.

Reconstitution

Before opening, it is recommended to briefly centrifuge the vial to settle the contents at the bottom. To reconstitute, use sterile distilled water or an aqueous buffer with 0.1% BSA, achieving a concentration of 0.1-1.0 mg/mL. Divide the stock solutions into working aliquots and store them at -20 °C or below. For further dilutions, use suitable buffered solutions.

Stability & Storage

To maintain the stability of this lyophilized preparation, it is recommended to store it at 2-8 °C. However, for long term storage, it should be kept at -20 °C and preferably in a desiccated form. Once reconstituted, the preparation can remain stable for up to one week at 2-8 °C. For optimal stability, it is advised to divide the reconstituted preparation into working aliquots and store it at -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze-thaw cycles to ensure its effectiveness.

Target

Accession

Q6UW88

Gene IDs

255324

References

References

1. Strachan L, Murison JG, Prestidge RL, et al. 2001. J Biol Chem, 276: 18265-71.

2. Kochupurakkal BS, Harari D, Di-Segni A, et al. 2005. J Biol Chem, 280: 8503-12.

3. Sahin UandBlobel CP. 2007. FEBS Lett, 581: 41-4.

4. Dahlhoff M, Muller AK, Wolf E, et al. 2010. J Invest Dermatol, 130: 623-6.

5. Taniguchi K, Yamamoto S, Aoki S, et al. 2011. Exp Lung Res, 37: 461-70.