Recombinant Human Epiregulin #abs00902

abs00902-5ug

1-2 Weeks

138

abs00902-25ug

1-2 Weeks

344

abs00902-1mg

1-2 Weeks

3891

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4 was filtered through a 0.2 μm filter and then lyophilized. Let's generate a highly similar content by rearranging the information provided.

Properties

aa_sequence

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Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

As per the LAL method, the Recombinant HumanEpiregulin has been found to possess less than 1 EU/μg. To state the same information in a different way, the determined level of Recombinant HumanEpiregulin as measured by the LAL method is below 1 EU/μg.

Activity

The standard and the ED show equal levels of biological activity, indicating their full effectiveness.₅₀The specific activity of the substance, which was assessed using murine Balb/c 3T3 cells through a cell proliferation assay, is greater than 5.0 × 10, with a corresponding concentration of less than 2 ng/ml. To put it differently, the concentration required to induce cell proliferation in Balb/c 3T3 cells is extremely low, while the specific activity of the substance is remarkably high.⁵ IU/mg.

Reconstitution

To ensure optimal results, we suggest a brief centrifugation of the vial prior to opening, to bring the contents to the bottom. Reconstitution should be done using sterile distilled water or an aqueous buffer containing 0.1 % BSA, with a target concentration of 0.1-1.0 mg/mL. It is recommended to divide the solution into working aliquots and store them at ≤ -20 °C. Dilutions should be made in appropriate buffered solutions. Follow these instructions to achieve the best possible outcome.

Stability & Storage

To maintain the stability of this lyophilized preparation, it is recommended to store it at 2-8 °C, however, for long term storage, it should be kept at -20 °C and ideally desiccated. Once reconstituted, it can be stored for up to one week at 2-8 °C. For optimal stability, it is advisable to divide the reconstituted preparation into smaller portions and store them in the freezer at -20 °C to -70 °C. To avoid degradation, do not subject it to repeated freeze/thaw cycles.

Target

Accession

O14944

Gene IDs

2069

References

References

1. Xi QS, Qian XG, Zhou QW, et al. 2000. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai), 32: 295-8.

2. Kuntz E, Broca C, Komurasaki T, et al. 2005. Growth Factors, 23: 285-93.

3. Shirakata Y, Kishimoto J, Tokumaru S, et al. 2007. J Dermatol Sci, 45: 69-72.

4. Thuong NT, Hawn TR, Chau TT, et al. 2012. Genes Immun, 13: 275-81.