Recombinant Human Amphiregulin #abs00904

abs00904-10ug

1-2 Weeks

138

abs00904-50ug

1-2 Weeks

344

abs00904-1mg

1-2 Weeks

1945

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4 was filtered through a 0.2 μm filter and then lyophilized. A highly similar content can be created by rearranging the information provided in the original text.

Properties

aa_sequence

QNFCIHGECK KKPQNKTESE NTSVRVEQVV KGKNGKNRRN RKKKNPCNAE FYIEHLEAVT CCQQEYFGEC SMTKTHSMID SSLSKGGKNG 。
The above content can be rearranged as follows:
The KKPQNKTESE NTSVRVEQVV begins with QNFCIHGECK, followed by KGKNGKNRRN, RKKKNPCNAE, FYIEHLEAVT, CCQQEYFGEC, and SMTKTHSMID. The content ends with SSLSKGGKNG.

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method was used to determine that Recombinant Human Amphiregulin has less than 1 EU/μg. It is important to ensure that any generated content accurately reflects this information while using unique language and phrasing.

Activity

Completely equivalent in biological activity to the reference standard. The effective dose (ED) is identical to that of the standard.₅₀According to a cell proliferation assay conducted with murine Balb/c 3T3 cells, the effective concentration of our product ranges from 5 to 10 ng/ml. It is crucial to note the accuracy of our findings and their significance in identifying the optimal dosage for achieving the desired effect. These results validate the effectiveness of our product and its potential for use in a variety of applications. We take pride in our commitment to producing high-quality products that have a measurable impact and produce consistent results.

Reconstitution

We suggest giving the vial a quick spin beforehand to settle the contents at the bottom. Next, dissolve this in sterile distilled water or aqueous buffer that includes 0.1% BSA until it reaches a concentration of 0.1-1.0 mg/mL. It is best to divide the stock solutions into workable aliquots, and safekeep them at or below -20 °C. Dilutions should be made in the appropriate buffered solutions.

Stability & Storage

To keep this lyophilized preparation in optimum condition, it should be stored at 2-8 °C. However, for long term storage, it is advisable to keep it at -20 °C and ensure that it is desiccated. Once you have reconstituted the preparation, it can be stored for up to one week at 2-8 °C. To ensure maximum stability, it is best to separate the reconstituted preparation into working aliquots and store them at -20 °C to -70 °C. Avoid exposing the preparation to repeated freeze/thaw cycles as this can affect its stability.

Target

Accession

P15514

Gene IDs

374

References

References

1. Giusti C, Desruisseau S, Ma L, et al. 2003. Int J Cancer, 105: 769-78.

2. Solic NandDavies DE. 1997. Exp Cell Res, 234: 465-76.

3. Michalopoulos GKandKhan Z. 2005. Gastroenterology, 128: 503-6.

4. Eckstein N, Servan K, Girard L, et al. 2008. J Biol Chem, 283: 739-50.

5. Higginbotham JN, Demory Beckler M, Gephart JD, et al. 2011. Curr Biol, 21: 779-86.