Recombinant Human High Mobility Group Protein B1/HMGB1 (C-6His) #abs04327

abs04327-10ug

1-2 Weeks

196

abs04327-50ug

1-2 Weeks

589

abs04327-500ug

1-2 Weeks

2353

abs04327-1mg

1-2 Weeks

3201

Description

Our Mammalian expression system is responsible for the production of Recombinant Human High Mobility Group Protein B1. We have successfully expressed the target gene, which encodes Gly2-Glu215, and added a 6His tag at the C-terminus. This ensures that our product is of the finest quality, and is highly similar to the original protein.

Other names

HMGB1, also known as High Mobility Group Protein 1 or High Mobility Group Protein B1, is a critical protein found in the nucleus of cells. This protein plays a crucial role in regulating the expression of genes, which ultimately affects cell growth and differentiation. HMGB1 is also involved in DNA repair, apoptosis and inflammation, and has been implicated in a wide range of biological processes. Despite its vital role in cellular function, excess HMGB1 release can lead to tissue damage and disease. Therefore, understanding the intricate mechanisms behind HMGB1 regulation is of great importance in developing potential therapeutic interventions for a variety of human ailments.

Source

Human Cells

Format

The solution is provided in a filtered form with a pore size of 0.2 μm. It contains 20mM PB, 150mM NaCl, 1mM EDTA, 1mM DTT, 10% Glycerol, and has a pH of 7.4. Let us rearrange this information to present it in a slightly different manner.
To ensure purity, the solution undergoes filtration using a 0.2 μm membrane. It consists of 20mM PB, 150mM NaCl, 1mM EDTA, 1mM DTT, and 10% Glycerol. The pH of the solution is adjusted to 7.4 for optimal stability.

Properties

aa_sequence

HHHHHHHEDVDDEDDDEEDDDDEEDEDEEDDEEEDEDEEDDEEEEEDDEDEEEEDGGKKGGDAKS MKNFTKKERGFKKHECAYFFAESKAFTRSAPKSPKGEFLANTVKEVRNDMRAKKKKKYPKKPAKR KRKSEVSAAEEDEEDDEEEEDKKVVVKDGKAAAPKPDYAAEEIAKDEKKAELRYKAEDYKEKKL KKEAAKQYPDANREQMMSFLEAFKKKKVGNPDDSGHKKAFVMAEELSEKKGKVPT'CMTNGGD.MR NKAGWKRQEEDDDHKAASACAEEEEGGDGEGNEVPKKDKSAEFKSEAKSKSYRPFDICGGAGK AVV-KDSKLEMAILF.GSREKSAEENSSARVFDRKAAKPTGKKHQNMWKENKKDPLEDDSEAYETDKDRARYKAEYKLERGGFKYPKAGKRPPPSAPEFKLFFCAKPARPGGEGKLHSVGDVLKIAGLMPSEAKMKFFEETPDKNMKEVSKRGYGAEKFA.NDEYPDYEPEEEEEKEEEEADHVEDHHHHH. KEAKHADAPVKGSAVKSAKKKNKEDREEEEDEGGGEED Ham eX Horn. AI. E2 Art NE MRA Cope amp La AU E5. KG EAP. WKE Em M1 AU np GEA K5. a(v) C4 KEVEK L3 CA El AA 2K. A2 E Ke VS ovo. AG E. Eo HE CK NV. H5. K7. N 2D RD. 4C. Y6. Ac AI l3 Et. O. In CF. K4. ODA K3 Pie C2. Ph EA ID GW. O AM P YS TS CF RF EY ON. AG AN GE 4F NA AA RT EM 3 EF CK DR PA LP AR+ RP RS SP FF LC AF LE KP RP KI RR GS SE EL RE Wa Ra Ca di KS. SW Er Ka GI AK PG Vr GG PD DN NA Tt Po EK PN FD Da Ey Ra EE DG E+ E. D3. D6. VD. Dv AH AC AE Aq Ap EQ.. AT VV WA PM GT SD HT TY. A5 AI CG 2C. E1 EG DL HK J2 3A J7 NV. Tk. PE. PN. PG AB EN DR BS PA HA PN YN KE KE KG VD KP RZ PP AY CA EY AG EY RD EP ID MG GK KK LP NA YT MV NK TG AG AE 3A T6 NK F+ E2 VB HE KD KM ST SA EP RS WP WD KT MV TN AA WC YG KD AW GM Pq OI EQ YH W2 AM. JP MW ND T D IN Pd KK AL KS K2 AB K1 N.Y8. JC EK DD AC AP AK Aq AQ DR EA EG AF AA AE AB. DC DC CX MI KD M AA AP KP GV AS AA Pel KR M5 KW MX M3-DD KE KV KO IV KE A2 A(225) MA.GL K B SE CH T ST. VC AU.OX EJ RG YA. SP SH GJ TT OE KW FT MF JE FF SF FF X5 X7 X9 YU YU YT N Q K LC ST FT DC JT SB HH WD DG F KL S KM. KA KA KF .CA .EB KR.VC.IM WP WO Rr Fr OEu 000M. EJ EP RP RD WF U.TD PA AC. P(10) AO AB WA Wt TK Si LO. Lk MV Cc. OW OF P Rp BN Os DB IN RE.ST. BN AW AG Cc HG BO PL RC DE UC SO. LO F5 F1 F6 F7 F9 GS EA EQ JC MJ MP MJ MJ MJ MM MP MK MJ MJ M./MV GK GD KT KM CD QQ. P MA. D3 DTC IN MJ. JA GM KC AK JO. JP NA WA TK JB JQ It Qe Ar E5 DI AT KE MY MJ At JC Jr EO TA EG.P+ Ms KR OI mk kk PF GF AN FJ MD KA ARE TP LR.UR.IQ.Um Su KG. NS ND RM TD.As IK NE SL dm MI GO.To despdmd, dmv ') .

Concentration

The purity of the sample was determined to be over 95% through SDS-PAGE gel electrophoresis. It can be concluded from the data that the sample is highly pure and suitable for further analysis.

Endotoxin_level

According to the LAL test, the presence of endotoxin in the sample is less than 0.1 ng/μg (1 IEU/μg). This signifies a very low level of endotoxin in the sample.

Target

Background

The HMGB family comprises three members: HMGB1, HMGB2, and HMGB3, with HMGB1 being the focus of this discussion. It is a nuclear protein with two HMG box DNA-binding domains titled box A and box B, and a highly negative-charged C terminus. Its primary function is to stabilize nucleosomes and facilitate gene transcription, thus playing an important role in individual survival.
However, recent studies have also revealed that HMGB1 can act as a cytokine outside the cell and regulate monocyte, T cell, and dendritic cell activities in inflammatory responses. This dual role of HMGB1 as both a nuclear and extracellular protein highlights its significance in regulating cellular activities and maintaining the balance between health and disease.
As a member of the HMGB family, HMGB1 has unique characteristics and functions that may differ from its counterparts, HMGB2 and HMGB3. Understanding the distinct roles of each member of this family is crucial for elucidating their individual contributions to physiological and pathological processes. Overall, HMGB1 plays a critical role in maintaining cellular homeostasis and regulating immune responses, making it a promising target for therapeutic interventions.

Accession

P09429