Recombinant Human OSM, 209a.a. #abs00858

abs00858-2ug

1-2 Weeks

138

abs00858-10ug

1-2 Weeks

344

abs00858-1mg

1-2 Weeks

6742

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4 was filtered through a 0.2 μm filter and subsequently lyophilized. Let me provide you with a rephrased version that maintains the original meaning but has a different structure: "After concentrating, the solution in PBS at pH 7.4 went through a 0.2 μm filtration process before undergoing lyophilization."

Properties

aa_sequence

RVPQGLQKQL TGCDMQRSLD IPYIRLQGLD REVKLPHCER EFAGPSELTG RRLGGRFLTQ NATLCLVHRL ARDLEQRLPQ ADLERSGNIE DLKEMQRLPA NIGLRNNIYC MAQLLDNSTA EPTKRGASAG QPPAPTSADF KRLEGCFRLH YHRFMHGVSF SKWGERPNSR RHSPHQALKR GVRRPSDALF QKIENGCSYA ALNIISHYWI QRWIRQKLFW VTDGTPSGIS DNFVEKYGNY LRFNSEVEID STYYVYSTSV LVSYYYERDS NSYYKHMNFS YRIAQIAKDG ELWAGTESWA AYYIDHYDEA EGPSSTEDLN LLLLLLDKWA KEPPKLEEEQ KQPPKSLEGA SYLQKTIERM GVAKGDTIYG MPPYDLRPPG TRNASYVAWK MAEAARYSTE ANTGTRPMAI GIPLPPPDPT EPLQLAETLE ELYSFQDYIS HLVEPHIRTL SHLA.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined that the Recombinant HumanOSM, with a length of 209a.a., contains less than 1 EU/μg. It's possible to create highly similar content by rearranging the above information, but using completely different language structures.

Activity

The standard and the fully biologically active versions were compared, and the latter was found to be equivalent in potency. The ED value of the two was identical, confirming the bioactivity of the fully active version. The similarity between the two indicates that the active properties of the standard version have been fully retained in the biologically active version.₅₀The specific activity of a protein, which was determined through a cell proliferation assay using human TF-1 cells, is greater than 5.0 × 10, with a threshold of less than 2 ng/ml. This indicates that the protein is highly effective and has potent activity in promoting cellular proliferation.⁵ IU/mg.

Reconstitution

To ensure proper mixing, we recommend a brief centrifugation of the vial before opening it. You can then reconstitute the contents with either sterile distilled water or an aqueous buffer that contains 0.1% BSA, aiming for a concentration between 0.1-1.0 mg/mL. If you're preparing stock solutions, divide them into smaller working aliquots and store them at temperatures equal to or below -20 °C. Any further dilutions should be done in suitable buffered solutions.

Stability & Storage

This freeze-dried product is able to withstand temperatures of 2-8 °C, yet extended storage should be carried out at -20 °C, preferably in a dry atmosphere. Upon reconstitution, it can be used steadily for one week if maintained at 2-8 °C. To ensure maximum stability, divide the reconstituted product into smaller, usable portions and store at -20 °C to -70 °C. Avoid the detrimental effects of repeating freeze/thaw cycles.

Target

Accession

P13725

Gene IDs

5008

References

References

1. Tanaka M, Miyajima A. 2003. Rev Physiol Biochem Pharmacol. 149:39-52.
2. Mosley B, De Imus C, Friend D, et al. 1996. J Biol Chem. 271:32635-43.
3. Malik N, Kallestad JC, Gunderson NL, et al. 1989. Mol Cell Biol. 9:2847-53.
4. Brown TJ, Rowe JM, Liu JW, et al. 1991. J Immunol. 147:2175-80.