Recombinant Human EMAP-II #abs00861

abs00861-5ug

1-2 Weeks

138

abs00861-20ug

1-2 Weeks

344

abs00861-1mg

1-2 Weeks

5058

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4, was filtered through a 0.2 μm filter and then lyophilized.

Properties

aa_sequence

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Concentration

> 98 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined that the Recombinant Human EMAP-II contains less than 1 EU/μg.

Activity

When compared to the standard, the ED is fully biologically active.₅₀The concentration of the protein that induces apoptosis in serum-free human MCF-7 cells is below 40 ng/ml, indicating a specific activity of greater than 2.5 × 10. This data serves as a reliable indicator of the protein's potency in inducing apoptosis in cancer cells. Researchers can use this information to effectively design and evaluate potential cancer therapies that target this protein. The ability to accurately measure and quantify the protein's apoptotic effect is essential for developing innovative treatments that can help combat cancer and improve patient outcomes.⁴ IU/mg.

Reconstitution

To ensure that the contents of this vial are evenly distributed at the bottom, we highly recommend a short centrifugation prior to opening. Reconstitution can then be achieved by diluting in sterile distilled water or an aqueous buffer containing 0.1% BSA, with a target concentration of 0.1-1.0 mg/mL. For storage, we suggest dividing the stock solution into smaller, working aliquots and keeping them at a temperature of ≤ -20°C. Any further dilutions should be made in the appropriate buffered solutions.

Stability & Storage

For optimal storage conditions of this lyophilized preparation, it is advised to keep it at a temperature range of 2-8 °C. However, for long-term storage, it should be stored at -20 °C, preferably in a desiccated environment. Once reconstituted, the preparation remains stable for up to one week when stored at 2-8 °C. To ensure maximal stability, it is recommended to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles as it can compromise its stability.

Target

Accession

Q12904

Gene IDs

9255

References

References

1. Awasthi N, Schwarz MA, Schwarz RE. 2010. Cancer Biol Ther, 10: 99-107.

2. Journeay SandSingh B. 2007. Acta Neuropathol, 114: 435; author reply 7-8.

3. Clarijs R, Schalkwijk L, Ruiter DJ, et al. 2003. Invest Ophthalmol Vis Sci, 44: 1801-6.

4. Kayton MLandLibutti SK. 2001. Curr Opin Investig Drugs, 2: 136-8.