Recombinant Mouse B7-H4 #abs04568

abs04568-10ug

1-2 Weeks

138

abs04568-50ug

1-2 Weeks

382

abs04568-500ug

1-2 Weeks

1711

abs04568-1mg

1-2 Weeks

2328

Description

Our Mammalian expression system is used to produce Recombinant Mouse B7 Homolog 4. The target gene is expressed with a Fc tag at the C-terminus, and it encodes Leu25-Ser256.

Other names

The V-set domain encompasses various proteins, including T-cell activation inhibitor 1, B7 homolog 4, Protein B7S1, and B7-H4, also known as VTCN1. These proteins play significant roles in regulating immune responses. By rearranging the content above, we can present the information in a different manner while maintaining its original meaning.

Source

Human Cells

Format

The pH7.4 PBS solution was filtered through a 0.2 μm filter and then lyophilized.

Properties

aa_sequence

TLTSIHTSFFSSGAGKKADELFEDkvPWVKTSFPIVLIYDCGPADRYQGREQMRSYQNRETESIKRVAMVQFYNYANPELIVEFPKSKESNVNGYEIPDKCIIAPNHGEQEVPLEESDYDRGKEYTRDNSSSQTKPQDTEKNLYKDVICTDIHRGKDFFFSWPGAASDKSLQNQWYEGYDPEPQKTSVTESSYNLQQVFFPYRKRAEPMWLKQTIKGKPFFTWGSEINEHTKYAVVTVIQHVEPTLAPEMTNQTGNWEDSFDTKEDHSAVLEEVFWTTLES.MNGIEKAPKIKHEIEQL-EDEKSFNQSTCSTCTKPYINRQSYFYNKGIVTEQKQRKLAVNKHESNDYWPPKITTI.QNILTLIONVLSCQNDDIQLVTQKSLENWVQSPGLNETPYQPPGQWKWYASYSCWRLSQDGVST.GLQFEKESVDPNVNVKYRRGKKAEQTT.PKCNQWEENSMRSLGGCVKHKSLFAQKRTQSNTLPRIQSNTYQGQTFGENSEFDK.DQKMNKSQDTEFLHGNSGEGKFFLQEKLELT.AvFLQGESNMKKKHDSLSPVPPKKPDGGEGQTDYQETPSLY.KIETPTAKNVYENNQEWGADWIHYFQVDGIKQG.

Concentration

To evaluate the protein concentration, SDS-PAGE analysis was performed, resulting in a determination of greater than 95%. This high percentage indicates a substantial amount of protein present in the sample.

Endotoxin_level

LAL1 IEU/μg（0.1 ng/μg）。，。

Reconstitution

Remember to always centrifuge tubes before opening to ensure that the contents are properly settled. Avoid using vortex or pipetting techniques for mixing as they may disrupt the integrity of the solution. It is crucial not to reconstitute the protein to a concentration lower than 100 μg/ml as it may affect its stability and activity. Use ddH2O to dissolve the lyophilized protein, taking care to aliquot the reconstituted solution to minimize the number of freeze-thaw cycles. By following these instructions, you can maintain the quality and effectiveness of the protein sample.

Target

Background

Mouse V-set domain-containing T-cell activation inhibitor 1/VTCN1/B7-H4 is glycosylated member of the B7 family of immune co‑stimulatory proteins. B7-H4 consists of extracellular domain (ECD) with one Ig-like V-set domain and one Ig-like C2-set domain. It is widely expressed, including in kidney, liver, lung, pancreas, placenta, prostate, spleen, testis and thymus. B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. It also involved in promoting epithelial cell transformation.

Accession

Q7TSP5