Recombinant Human Dickkopf-Related Protein 1/DKK1 (N-8His) #abs04355

abs04355-10ug

1-2 Weeks

230

abs04355-50ug

1-2 Weeks

673

abs04355-500ug

1-2 Weeks

2353

abs04355-1mg

1-2 Weeks

3491

Description

Our Mammalian expression system is responsible for producing Recombinant Human Dickkopf-related protein 1. The target gene that is expressed includes a 8His tag at the N-terminus and encodes Thr32-His266. So, to summarize, our system generates the recombinant protein by expressing the specific target gene along with the required 8His tag.

Other names

Dickkopf-related protein 1, Dickkopf-1, Dkk-1

Source

Human Cells

Format

The solution of PBS with a pH of 7.4 was filtered through a 0.2 μm filter and then lyophilized to create the final product.

Properties

aa_sequence

NSVYLGGNKYPQYPCTDNYQEEDEECGSPTRGGDAQICLACVQNHFRGEIEEKRCRPAMHSCMGPN YCKNGRHTLSSDGYSSTLDQTIDNVSAAPGPGSAVPPPLNLPPPKNAIKNPPLGGAAGHPGQTL HHHHHHHHSCLACRKRRKRCMRESFGNDASGLCCARRKGQVCTKHRRKGSVLCRCYCGESDCARH FWSKEIGSHGLEIFQKDHEECASNSSRLHTCQRH。，。

Concentration

Ascertained through the employment of reducing SDS-PAGE, the level of similarity was found to be above 95%. It is possible to create similar content by rearranging the aforementioned sentence while ensuring that the generated content is based on the original information.

Endotoxin_level

The result of the LAL test indicates that the concentration is below 0.1 ng/μg (1 IEU/μg). To put it differently, the determination from the original content highlights that the level of this particular substance is extremely low, as confirmed by the LAL test.

Reconstitution

To ensure accurate results, it is important to always centrifuge tubes before opening and avoid mixing the protein by vortex or pipetting. It is also advisable not to dilute the protein to a concentration lower than 100 μg/ml. When reconstituting the lyophilized protein, use ddH2O and consider aliquoting the solution to minimize freeze-thaw cycles. These steps will help maintain the integrity of the protein and ensure optimal performance.

Stability & Storage

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target

Background

Dickkopf-related protein 1 (DKK-1), is a member of the dickkopf family. DKK1 secreted proteins with two cysteine-rich domains separated by a linker region. It antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

Accession

O94907