Recombinant Rat MIF #abs01307

abs01307-10ug

1-2 Weeks

138

abs01307-50ug

1-2 Weeks

344

abs01307-1mg

1-2 Weeks

3079

Species

Rat

Source

Escherichia coli.

Format

A concentrated solution in 20 mM Tris pH 8.0, 150 mM NaCl, 3% trehalose was filtered through a 0.2 μm membrane to yield a lyophilized product. The composition of the product is highly similar to the original solution, maintaining the pH, buffer system, salt concentration, and stabilizing agent. This dry form allows for ease of storage and transportation without compromising the stability and activity of the initial solution.

Properties

aa_sequence

GAQNRNYSKL LVNTNVPRAS VPEGFLSELT QQLAQATGKP AQYIAVHVVP DQLMTFSGTS DPCALCSLHS IGKIGGGGST SDRLHISPDR VYINYYDMNA ANVGWNSFTG SHTKTTVQM。

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined that rRtMIF contains less than 1 EU/μg. A highly similar content can be generated by rearranging the words and phrases: The LAL method was utilized to ascertain that rRtMIF has a measly amount of less than 1 EU/μg.

Activity

Test in process.

Reconstitution

It is advisable to briefly centrifuge the vial before opening it, in order to settle the contents at the bottom. The reconstitution process involves adding sterile distilled water or an aqueous buffer with 0.1 % BSA to achieve a concentration of 0.1-1.0 mg/mL. To maintain the integrity of the stock solution, it should be divided into smaller working aliquots and stored at temperatures equal to or below -20 °C. Any further dilutions should be prepared using suitable buffered solutions.

Stability & Storage

For optimal storage conditions, it is recommended to keep this lyophilized preparation at -20 °C. However, short-term storage at 2-8 °C is also acceptable. To enhance its stability, it is advisable to desiccate the preparation. Once reconstituted, it remains stable for approximately one week when stored at 2-8 °C. To maximize its shelf life, it is suggested to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles.

Target

Accession

P30904

Gene IDs

81383

References

References

1. WY Weiser, PA Temple, JS Witek-Giannotti, et al. 1989. Proc Natl Acad Sci U S A, 86: 7522-6.

2. CA Kozak, MC Adamson, CE Buckler, et al. 1995. Genomics, 27: 405-11.

3. DF LarsonandK Horak. 2006. Crit Care, 10: 138.

4. HW Sun, J Bernhagen, R Bucala, et al. 1996. Proc Natl Acad Sci U S A, 93: 5191-6.

5. M Oddo, T Calandra, R Bucala, et al. 2005. Infect Immun, 73: 3783-6.

6. M Emonts, FC Sweep, N Grebenchtchikov, et al. 2007. Clin Infect Dis, 44: 1321-8.