Recombinant Human Wnt Inhibitory Factor 1/WIF-1 (C-6His) #abs04332

abs04332-10ug

1-2 Weeks

123

abs04332-50ug

1-2 Weeks

337

abs04332-500ug

1-2 Weeks

2353

abs04332-1mg

1-2 Weeks

3201

Description

Our Mammalian expression system is responsible for the production of Recombinant Human Wnt Inhibitory Factor 1. The target gene, which encodes Gly29-Trp379, is expressed with a 6His tag at the C-terminus.

Other names

Wnt Inhibitory Factor 1, WIF-1, WIF1

Source

Human Cells

Format

The solution with 10mM HAc-NaAc, 150mM NaCl, and 0.5% CHAPS was filtered through a 0.2 μm filter and then lyophilized, with the pH maintained at 4.0 throughout the process. The resulting product contains all of the same components as the original solution and is highly similar in composition.

Properties

aa_sequence

VPQNNVMSHKDILAEGKYLIFESLDWPIGQRAAHWMPTFEKFQRDPAAQMTIVPHNSWGFEVNTWQA GYLEEFQYSEFLSRDLRLSIGTNADPPVVLNVPHTKSLVGQAFGPCLGEDQAVAFIVEDVMNSI SEGKITLNMQANAFFKTCQACEPCGGRNCGFECEPNCRICERIFHGHGPCMKALTFPGDVCNVGL CVCPTGPFICPPGYFGVNCDNKTSTCCFNGTCCYYGKPICPPGEEGLECRINSKCGPHGPCMNGK GYQGDLCSKSKCEPGCGAKGTCHEPNKCQCQHGWHGRHCKNKYEASLHALRAQPLRQHTLKKAES RDRPEESINYIHQQQQQQQ.

Concentration

SDS-PAGE，95%。，。

Endotoxin_level

The LAL test has determined that the level of endotoxin in this sample is less than 0.1 ng/μg or 1 IEU/μg. It is important to maintain a low level of endotoxin in order to ensure the safety and effectiveness of the product.

Reconstitution

To ensure proper handling and preparation of the lyophilized protein, it is essential to follow certain guidelines. Firstly, always make sure to centrifuge the tubes prior to opening them. It is highly recommended not to mix the contents using vortex or pipetting techniques. Additionally, it is not advisable to dissolve the protein to a concentration lower than 100 μg/ml.
To reconstitute the lyophilized protein, use ddH20 and ensure that you aliquot the solution to reduce the number of freeze-thaw cycles. It is also crucial to remember that the reconstituted solution should have a highly similar composition to its original state. Therefore, be sure to follow all the steps described in the guidelines to obtain the best possible results.

Target

Background

Wnt Inhibitory Factor 1 (WIF1) is a secreted protein, which binds WNT proteins and inhibits their activities. WNT proteins are extracellular signaling molecules involved in the control of embryonic development. WIF1 contains a WNT inhibitory factor (WIF) domain and 5 epidermal growth factor (EGF)-like domains. is found to be present in fish, amphibia and mammals. WIF1 is a recurrent target in human salivary gland oncogenesis.WIF1 may be involved in mesoderm segmentation. WIF1 is a tumor suppressor, specifically in nonfunctioning pituitary tumors.

Accession

Q9Y5W5