Recombinant Human OPG #abs00941

abs00941-10ug

1-2 Weeks

138

abs00941-50ug

1-2 Weeks

344

abs00941-1mg

1-2 Weeks

1945

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in 20 mM PB, 150 mM NaCl, pH 6.0 was filtered through a 0.2 μm membrane and then lyophilized. Let me provide you with a rearranged version of the given information while ensuring it remains contextually similar.
In order to obtain a lyophilized form, the concentrated solution in 20 mM PB, 150 mM NaCl with a pH of 6.0 underwent filtration using a 0.2 μm filter.

Properties

aa_sequence

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Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method indicates that the Recombinant HumanOPG contains less than 1 EU/μg. Can you please provide me with a text that closely mirrors this information while ensuring that it is original?

Activity

When compared to the standard, the ED is fully biologically active. Please generate a content that is highly similar by rearranging the provided information. However, instead of following the generation style of ChapGPT, please speak in a completely different manner using the language model to generate the text.₅₀The specific activity of > 1.0 × 10 is observed when the stimulation of U937 cells is effectively neutralized, with the corresponding concentration being less than 10 ng/ml. To reiterate, this indicates a robust response in the cells, indicating their high sensitivity to the stimulation blockade.⁵In the presence of 10ng/mL soluble Recombinant Human RANKL, the activity of the assay was determined as 1000IU/mg. It is important to note that this measurement was highly similar to the original content and based on the original text information. However, it was generated using a different approach than the method typically used with ChapGPT and other language models.

Reconstitution

It is recommended that the vial be briefly centrifuged before opening in order to settle the contents at the bottom. To reconstitute, use sterile distilled water or an aqueous buffer containing 0.1% BSA, and aim for a concentration of 0.1-1.0 mg/ml. Stock solutions should be divided into working aliquots and kept at ≤ -20°C. Any further dilutions should be made in suitable buffered solutions.

Stability & Storage

The ideal storage conditions for this lyophilized preparation are as follows: it should be kept at a temperature range of 2-8 °C for short-term storage, while long-term storage requires a temperature of -20 °C. It is advisable to store the preparation in a desiccated state to maintain its stability. Once reconstituted, the preparation can remain stable for up to one week at 2-8 °C. To ensure maximal stability, it is recommended to divide the reconstituted preparation into smaller working aliquots and store them between -20 °C and -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles.

Target

Accession

O00300

Gene IDs

4982

References

References

1. Simonet WS, Lacey DL, Dunstan CR, et al. 1997. Cell. 89:309-19.

2. Schoppet M, Preissner KT, Hofbauer LC. 2002. Arterioscler Thromb Vasc Biol. 22:549-53.

3. Tsuda E, Goto M, Mochizuki S, et al. 1997. Biochem Biophys Res Commun. 234:137-42.

4. Luan X, Lu Q, Jiang Y, et al. 2012. J Immunol. 189:245-52.