Recombinant Human PTH1-84 #abs01217

abs01217-20ug

1-2 Weeks

138

abs01217-100ug

1-2 Weeks

344

abs01217-1mg

1-2 Weeks

1702

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4 was filtered through a 0.2 μm filter and then lyophilized.

Properties

aa_sequence

KASQDLTVNV NADKAEGLSK ELSKHESKVE VLQKLRWLE VEMSNLHKLG NHLMQIESVS RQPDSAAGPL PAGALVFNHV DQKLPPRERN VKDNEKRPKR.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined that the Recombinant HumanPTH1-84 contains less than 1 EU/μg. In order to generate a similar content, the information in the original text can be rearranged and presented in a different way. For instance: Based on the LAL method, the level of endotoxins in the Recombinant HumanPTH1-84 is less than 1 EU/μg. This confirms the high quality of this product for use in scientific research and medical applications.

Activity

The standard's biological activity is completely comparable to that of the ED.₅₀The specific activity of the protein, as measured by its ability to stimulate cAMP accumulation in MC3T3E1 cells, is greater than 2.0 × 10, corresponding to a potency level of less than 50 ng/ml. This indicates that the protein is highly potent and effective in its biological activity, making it a valuable tool for research and therapeutic applications. The MC3T3E1 cell line is commonly used for studying bone formation and development, and the protein's ability to induce cAMP accumulation in these cells suggests that it may have important roles in bone biology. Further studies are needed to fully understand the mechanisms by which this protein exerts its effects and to explore its potential applications in disease treatment.⁴ IU/mg.

Reconstitution

To ensure optimal use of this vial, it is recommended to briefly centrifuge it before opening so that the contents settle at the bottom. Reconstitute the product in sterile distilled water or aqueous buffer, with the addition of 0.1% BSA, at a concentration of 0.1-1.0 mg/ml. Once prepared, stock solutions should be divided into appropriate working aliquots and stored at -20°C or lower. Dilutions should be made in suitable buffered solutions.

Stability & Storage

For optimal storage, it is recommended to keep this lyophilized preparation at -20 °C. However, it can also be stored at 2-8 °C without compromising its stability. In order to maintain its long-term stability, it is advisable to store it at -20 °C while ensuring it is desiccated. Once reconstituted, the preparation can remain stable for up to one week when stored at 2-8 °C. To enhance its stability even further, it is recommended to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to prevent the formation of ice crystals by avoiding repeated freeze/thaw cycles.

Target

Accession

P01270

Gene IDs

5741

References

References

1. Potts, J.T. 2005. J Endocrinol, 187: 311-25.

2. Misiorowski, W. 2011. Endokrynol Pol, 62: 73-8.

3. Scillitani, A., V. Guarnieri, C. Battista, et al. 2011. J Endocrinol Invest, 34: 23-6.