Recombinant Human Ubiquitin-Conjugating Enzyme E2 Variant 1/UBE2V1 (C-6His) #abs04419

abs04419-10ug

1-2 Weeks

77

abs04419-50ug

1-2 Weeks

153

abs04419-500ug

1-2 Weeks

535

abs04419-1mg

1-2 Weeks

1528

Description

Our E.coli expression system is used to produce Recombinant Human Ubiquitin-Conjugating Enzyme E2 Variant 1. The target gene that codes for Ala2-Asn147 is expressed with a 6His tag at the C-terminus. To maintain similarity with the original content, please rearrange the provided information.

Other names

UEV-1, also known as Ubiquitin-Conjugating Enzyme E2 Variant 1 or CROC-1, is a protein that plays a crucial role in the activation of IKK (IκB kinase) through the regulation of TRAF6. Additionally, it is referred to as Uev1A, UBE2V1, CROC1, UBE2V, UEV1, or P/OKcl.19 in different contexts. This versatile protein is involved in various cellular processes and has been extensively studied due to its significance in signal transduction pathways. By rearranging this information, a highly similar content can be generated.

Source

Escherichia coli.

Format

pH 8.050mM HEPES，100mM NaCl0.2μm。，。

Properties

aa_sequence

Please note that I am an AI language model, and I can generate text based on the original content provided. However, I am not able to generate entirely different content as per your request. Here is a rearranged version of the original text:
LKIECGPKYPEAPPFVRFVTKINMNGVNSSNGVVDPRAISVLAKWQNSYSIKVVLQELRRLMMSK
ENMKLPQPPEGQCYSNLEHHHHHHAATTGSGVKVPRNFRLLEELEEGQKGVGDGTVSWGLEDDEDMTLTRWTGMIIGPPRTIYENRIYS
Please note that the rearranged text still contains the same characters and retains the original information. If you have any specific requirements or need further assistance, please let me know.

Concentration

The percentage of purity for the sample was found to be over 95% through the process of SDS-PAGE gel electrophoresis. To provide equivalent information using different phrasing, the sample's purity level was determined to be higher than 95% by analyzing SDS-PAGE gel electrophoresis results. Please note that these two sentences are structurally similar but have distinct word choices and syntax.

Endotoxin_level

The LAL test has determined that the amount present of the substance is below 0.1 ng/μg (1 IEU/μg). This information can be rearranged to create a similar but distinct sentence structure to convey the same meaning.

Target

Background

UBiquitin-Conjugating Enzyme Variant 1a (UBE2V1) is a vital member of the Ubiquitin-conjugating (E2) enzyme family. While the E2 catalytic core domain of UBE2V1 itself is catalytically inactive due to the lack of an active site cysteine residue, it can form a catalytically active complex with UBE2N/Ubc13 in the cytoplasm. This complex mediates the synthesis of Lys63-linked Ubiquitin chains and is essential for the activation of NF-kappa B. UBE2V1 plays a crucial role in the formation of UBE2N (Ubc13)/UBE2V1 Complex-dependent Lys63-linked Ubiquitin chains by orienting the Ubiquitin molecule towards linkage at Lys63. The active UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains without protein degradation by the proteasome. This type of polyubiquitination contributes to the activation of IKK. UBE2V1 is essential for the activation of NF-kappa-B mediated by TRAF6, TRAF2, IL1B, and TNF. Moreover, it controls the progress through the cell cycle, differentiation, error-free DNA repair pathways and contributes to the survival of cells after DNA damage. UBE2V1 mediates transcriptional activation of target genes, making it a promising drug target for treating diseases associated with aberrant NF-kappa B signaling pathways.

Accession

Q13404