Recombinant Staphylokinase #abs01238

abs01238-20ug

1-2 Weeks

138

abs01238-100ug

1-2 Weeks

344

abs01238-1mg

1-2 Weeks

1135

Source

Escherichia coli.

Format

The concentrated solution in PBS with a pH of 7.4 was filtered through a 0.2 μm filter and then lyophilized. The resulting lyophilized product contains all the same properties as the original concentrated solution, but in a more stable form. This process ensures that the product is highly similar to the original solution and can be easily reconstituted as needed.

Properties

aa_sequence

EVPVTTFPDI LPYVMEKNTG ESKKDNKYKD PVGKDAISGF VYTKRPMVES EKEKWIDKRA GKYNPDVLEV AFPVSVIDKL SEDVKGKYEE EIKVNNKIPY GTKTVFHKLE SDKTITVVKY VALDKFERRK KKGTTDSYEP.Following content has been generated by rearranging the above content: "EVPVTTFPDI LPYVMEKNTG ESKKDNKYKD PVGKDAISGF VYTKRPMVES EKEKWIDKRA GKYNPDVLEV AFPVSVIDKL SEDVKGKYEE EIKVNNKIPY GTKTVFHKLE SDKTITVVKY VALDKFERRK KKGTTDSYEP"

Concentration

> 97% by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method has determined that the amount of endotoxins in rSAK is below 1 EU/μg. Can you please generate a text that closely resembles the original information by rearranging the words and phrases? It is important that the text is based on the given content.

Activity

The standard and the test sample both exhibit complete biological activity. The specific activity of the sample was determined through fibrin lysis in an agarose plate and found to be 5.0 × 10, indicating high similarity to the standard.⁴ IU/mg.

Reconstitution

To ensure optimal results, it is advisable to briefly centrifuge the vial before opening to settle the contents. The protein should be reconstituted in sterile distilled water or an aqueous buffer solution containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Stock solutions should be divided into working aliquots and stored at temperatures at or below -20°C. Subsequent dilutions should be made in the appropriate buffered solutions.

Stability & Storage

To ensure its stability, this lyophilized preparation can be stored at 2-8 °C, but for long-term storage, it is recommended to keep it at -20 °C, preferably in a desiccated state. Once reconstituted, the preparation remains stable for up to one week at 2-8 °C. To maintain its maximal stability, it is advised to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles.

References

References

1. Matsuo O, Okada K, Fukao H, et al. 1990. Blood, 76: 925-9.

2. Collen D, Schlott B, Engelborghs Y, et al. 1993. J Biol Chem, 268: 8284-9.

3. Shishido Y, Matsumoto T, Sakai M, et al. 1994. Biol Pharm Bull, 17: 1060-4.

4. Ohlenschlager O, Ramachandran R, Flemming J, et al. 1997. J Biomol NMR, 9: 273-86.