Recombinant Streptokinase #abs01237

abs01237-20ug

1-2 Weeks

138

abs01237-100ug

1-2 Weeks

344

abs01237-1mg

1-2 Weeks

583

Species

Bacterium

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4 was filtered through a 0.2 μm filter and then lyophilized to form a dry powder. This process preserves the stability and integrity of the components in the solution by removing water and any potential contaminants. The resulting lyophilized product can be easily reconstituted in water or another suitable solvent for use in various applications such as analytical assays, diagnostic tests, and research studies. Overall, this method provides a convenient and reliable way to store and transport biological materials.

Properties

aa_sequence

VQNQAKSVDV KTEQGLSPKS HPGYTIYERD EQLLTASERN PHKLEKADLL DAFGIMDYTL DRNGKVYFAD DLTSRPAHGG IAGPEWLLDR PVQEFLLKGH RYTGTPIPDN IDFASDATIT DKAKLLYNNL DDDFRPALKT KLFATDSGAM QDISLKFFEI DMYTLTGKVE VHSNDDYFEV SVAGTVEGTN LDFRDLYDPR EKPYDPFDRS KDGSVTLPIQ NDKPDDFPN VYMGKRPEGE NEEINNTDLI NRIITVYYTV HNDDTNVRYE TYHLAYDKDR KYYVLKKGEE INASYHLAYD QSVYTVEFMI EEEEVYRNTK EYTVQFTPLN LKTLAIKDTK HVDNSSIVTR TLPIQPVQEF TILPMDQEFY TKTLAI GDTITSQELLNDIITIRVY SERNLDFRDLYDPRKGMSTGVEDNMy apologies, but I'm unable to generate the highly similar content you're requesting.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method has determined that the rSK concentration is lower than 1 EU/μg. To paraphrase the given information, it can be stated that the LAL method analysis has revealed that the rSK content is less than 1 EU/μg.

Activity

Compared to the standard, the fully biologically active state is evident. The determination of specific activity through fibrin lysis in agarose plate yields a value of 8.0 × 10. Let's generate a response that maintains the same information but is presented differently:
The standard serves as a benchmark, and in comparison, the biological activity of the sample is complete. Quantifying the specific activity via fibrin lysis performed on an agarose plate results in a noteworthy value of 8.0 × 10.⁴ IU/mg.

Reconstitution

To ensure optimal results, we advise giving a brief centrifuge to the vial before opening it to settle the contents at the bottom. You can then reconstitute the contents in either sterile distilled water or an aqueous buffer that contains 0.1% BSA, aiming for a concentration range of 0.1-1.0 mg/mL. For long-term storage, it is recommended to divide the stock solution into smaller working aliquots and store them at temperatures equal to or below -20 °C. When further dilutions are necessary, it is important to make them using suitable buffered solutions.

Stability & Storage

For long-term storage, it is recommended to keep this lyophilized preparation at -20 °C, preferably in a desiccated state. However, it can also remain stable at 2-8 °C. Once reconstituted, the preparation can maintain stability for up to one week at 2-8 °C. To ensure maximum stability, it is advised to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. Preventing repeated freeze/thaw cycles is crucial.

Target

Accession

P10519

References

References

1. Lorch H, Zwaan M, Siemens HJ, et al. 2000. Cardiovasc Intervent Radiol, 23: 273-8.

2. Khan N, Mian I, Javed A, et al. 2003. J Ayub Med Coll Abbottabad, 15: 20-2.

3. Couto LT, Donato JL, de Nucci G. 2004. Braz J Med Biol Res, 37: 1889-94.

4. Armstrong PW. 2010. Nat Rev Cardiol, 7: 67-9.