Recombinant Human MIC-A, His #abs01258

abs01258-10ug

1-2 Weeks

138

abs01258-50ug

1-2 Weeks

344

abs01258-1mg

1-2 Weeks

3079

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4, and 8 M Urea was filtered through a 0.2 μm filter, after which it was lyophilized. To create content that closely resembles the original text, rearrange these details accordingly.

Properties

aa_sequence

GFLAEVHLDG DNSTRSSQHF YFQGAMDPEF DYDIPTTENL VLSWDGSVQS YYDGELFLSQ QPFLRYDRQK KDQKEGLHSL EPHSLRYNLT EDVLGNKTWD RETRDLTGNG KDLRMTLAHI HADCLQELRR TCRASSFYPR VLSHDTQQW NLETEEWTVP QSSRAQTLAM NVRNFLKEDA TVPPMVNVTR SEASEGNITV YYDYELFLSQ CRAKPQGQWA RDGVSLQHTW EEVQLWDMRP KTLSLEAQHI HMKGQKEWNL ONTTGLICQN TAHSCCRQYY SFDHYPRRNC GEVRVTFYIC HSPMTNHSEG PSLPVKVSGK.

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method has revealed that the amount of Recombinant HumanMIC-A, His is less than 1 EU/μg. A similar statement can be created by rearranging the same information: The level of Recombinant HumanMIC-A, His has been determined to be less than 1 EU/μg using the LAL technique.

Activity

The biological activity of the substance is equivalent to that of the standard. To determine the specific activity, ELISA is used to measure the binding of MICA antibody. A high degree of similarity can be achieved by reorganizing the original content.

Reconstitution

To ensure proper handling of this vial, we recommend a brief centrifugation prior to opening. This will help settle the contents at the bottom. To reconstitute, use sterile distilled water and aim for a concentration of 1.0 mg/mL. Once you have a stock solution, divide it into working aliquots and store at ≤ -20 °C. When making further dilutions, buffered solutions that contain 8 M Urea will be appropriate. Follow these instructions carefully to ensure maximum efficacy of the product.

Stability & Storage

To ensure the stability of this lyophilized preparation, it is recommended to keep it at 2-8 °C, but for long-term storage, it's best to store it at -20 °C while keeping it desiccated. Once reconstituted, the preparation can remain stable for one week if stored at 2-8 °C. For optimal preservation, divide the reconstituted preparation into separate working aliquots and store them at -20 °C to -70 °C, making sure to avoid any repeated freeze-thaw cycles.

References

References

1. Yao Z, Volgger A, Helmberg W, et al. 1999. Eur J Immunogenet, 26: 225-32.

2. Li P, Willie ST, Bauer S, et al. 1999. Immunity, 10: 577-84.

3. Petersdorf EW, Shuler KB, Longton GM, et al. 1999. Immunogenetics, 49: 605-12.

4. Komatsu-Wakui M, Tokunaga K, Ishikawa Y, et al. 1999. Immunogenetics, 49: 620-8.

5. Gambelunghe G, Ghaderi M, Cosentino A, et al. 2000. Diabetologia, 43: 507-14.