Recombinant Human MIF #abs01260

abs01260-10ug

1-2 Weeks

138

abs01260-50ug

1-2 Weeks

344

abs01260-1mg

1-2 Weeks

3079

Description

Macrophage Migration Inhibitory Factor is a protein produced naturally by the human body. However, Recombinant Human Macrophage Migration Inhibitory Factor is a form of this protein that is produced artificially. It is often used for research purposes, as it is highly pure and can be used in a variety of experiments. The goal is to create a protein that is as similar to the naturally occurring protein as possible, while also having certain modifications that allow for better stability, solubility, and purity. Overall, Recombinant Human Macrophage Migration Inhibitory Factor is an important tool in the study of human biology and disease.

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4, was filtered through a 0.2 um filter and then lyophilized.

Properties

aa_sequence

AAVHVVPDQLM AFSELTTQLAQ AGAQNR SYSKLLCG LLAERLRIS PD RVYINY YDM NANVG WNSTFA MP MFIVNTNV PR ASVPDG FLS GGSS EPCALCSLHSI GKIG T KPPQYIA VH.
The above content seems to be a sequence of amino acids in a protein. The specific protein and its function may require further investigation.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The Recombinant HumanMIF was found to have less than 1 EU/ug, as determined using the LAL method. Let me rephrase this information in a distinct manner.

Activity

The binding of rhCD74 in a functional ELISA determines the specific activity, making it fully biologically active compared to the standard. Can you please provide some additional context or details?

Reconstitution

Before opening the vial, it is recommended to briefly centrifuge it so that the contents settle at the bottom. To reconstitute, use sterile distilled water or an aqueous buffer with 0.1% BSA, aiming for a concentration of 0.1-1.0 mg/mL. To maintain stability, divide the stock solutions into working aliquots and store them at or below -20 °C. For further dilutions, use appropriate buffered solutions.

Stability & Storage

The lyophilized preparation can be stored at 2-8 °C, but for long-term storage, it is recommended to keep it at -20 °C, preferably in a desiccated state. Once reconstituted, the preparation remains stable for up to one week at 2-8 °C. To ensure maximum stability, it is advised to divide the reconstituted preparation into smaller portions and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles.

References

References

1. Edwards KM, Tomfohr LM, Mills PJ, et al. 2011. Sleep, 34: 161-3.

2. Delaloye J, De Bruin IJ, Darling KE, et al. 2012. Cytokine,

3. Leu RW, Woodson PD, Whitley SB. 1977. J Reticuloendothel Soc, 22: 329-40.

4. Landolfo S. 1977. G Batteriol Virol Immunol, 70: 137-43.

5. Baugh JA, Chitnis S, Donnelly SC, et al. 2002. Genes Immun, 3: 170-6.