Recombinant Human MIF, His #abs01261

abs01261-10ug

1-2 Weeks

138

abs01261-50ug

1-2 Weeks

344

abs01261-1mg

1-2 Weeks

3079

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4 was filtered through a 0.2 μm filter and then lyophilized to produce a powdered form. By rearranging the original content, this highly similar text conveys the same information in a slightly different structure.

Properties

aa_sequence

LPHAGQSPNSV TYPPGQDYLA VHQAVGNVFI RPQGSGKTLA LYVLLERINK FSYSLNSHAV HNAGGWWKDV CDGGLPYSRT PKQGSVFHMA PANVDELVCI SHCGKIYIGG LMRLPPFNST VYDNKHEMNA NAGWSSFHGA AAAAGGHHHP LYHASNSTFA LEEHVMMMM.Please create similar content by rearranging the given text, while ensuring that the content is based on the original text information but using a different approach of text generation.

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The concentration of Recombinant Human MIF, His was found to be below 1 EU/μg using the LAL method. I have rearranged the content to ensure it is based on the original information.

Activity

The standard and the fully active form of this substance were compared, and it was found that the specific activity of the active form is determined by its ability to bind to rhCD74 in a functional ELISA assay. Let's create a highly similar version of the content by rearranging the information provided above.

Reconstitution

Before opening, it is recommended to briefly centrifuge the vial to ensure that the contents settle at the bottom. To reconstitute, use sterile distilled water or an aqueous buffer with 0.1% BSA, targeting a concentration of 0.1-1.0 mg/mL. It is advisable to divide the stock solutions into working aliquots and store them at or below -20 °C. Any further dilutions should be prepared using appropriate buffered solutions.

Stability & Storage

For long-term storage, it is recommended to keep this lyophilized preparation at -20 °C, preferably desiccated. However, it can also be stored at 2-8 °C, remaining stable. After reconstitution, the preparation can be stored at 2-8 °C for up to one week. To ensure maximum stability, it is advised to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles, as this may affect its stability.

References

References

1. Edwards KM, Tomfohr LM, Mills PJ, et al. 2011. Sleep, 34: 161-3.

2. Delaloye J, De Bruin IJ, Darling KE, et al. 2012. Cytokine,

3. Leu RW, Woodson PD, Whitley SB. 1977. J Reticuloendothel Soc, 22: 329-40.

4. Landolfo S. 1977. G Batteriol Virol Immunol, 70: 137-43.

5. Baugh JA, Chitnis S, Donnelly SC, et al. 2002. Genes Immun, 3: 170-6.