Recombinant Human HMGB1, His #abs01262

abs01262-10ug

1-2 Weeks

138

abs01262-50ug

1-2 Weeks

344

abs01262-1mg

1-2 Weeks

3079

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4 was filtered through a 0.2 μm filter and subsequently lyophilized. To produce a content that closely resembles the original text, we can simply rearrange the above statement while preserving the essential information. Specifically, we can say that the lyophilized product was obtained by filtering a concentrated solution in PBS, pH 7.4 through a 0.2 μm filter.

Properties

aa_sequence

KDKMGEKSPR KFFLYSAFVQ ECQKMMRKKK HNDPAVSFKS SNKERWKGKA TAKSGMKFED MRDAKAAKDR EKKRYTMIEP PKGEKKKFFK DSPPARPPNK FFFSCYEAPL RKIKPEGPGS VIGDAKKKGL EMWNNTDAAD EPQKAEAKKA AKEEDDDDEE DEEDEDDDEE EDEDDDDDEE DEDEDEDEEE EDDEEEDDDD DDDLEEEHEE HHHHHHHHHH

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The recombinant human HMGB1, with a His tag, contains less than 1 EU/μg as determined by the LAL method. To create a similar content, one can rearrange the details provided in the given statement to convey the same information in a different manner without using ChapGPT generated content.

Reconstitution

Prior to opening this vial, it is recommended to briefly centrifuge its contents in order to settle them at the bottom. Reconstitute the contents in either sterile distilled water or an aqueous buffer containing 0.1 % BSA, to achieve a concentration of 0.1-1.0 mg/mL. Preparing stocks should be done by dividing aliquots and storing them at a temperature of ≤ -20 °C. Dilute further, if necessary, in suitable buffered solutions.

Stability & Storage

To ensure the stability of this lyophilized preparation, it should be stored between 2-8°C. However, for longer term storage, it is recommended to keep it at -20°C, and preferably desiccated. Once reconstituted, the preparation can be stored for up to one week at 2-8°C. For optimal stability, it is advisable to divide the reconstituted preparation into smaller working aliquots and store them between -20°C and -70°C. It is essential to avoid repeated freeze/thaw cycles as this may affect the preparation's quality and effectiveness.

Target

Accession

P09429

Gene IDs

3146

References

References

1. Abdulahad D, Westra J, Reefman E, et al. 2013. Lupus,

2. Li G, Liang X, Lotze MT. 2013. Front Immunol, 4: 68.

3. Tang J, Deng P, Jiang Y, et al. 2013. Cell Biol Int, 37: 262-6.

4. Zhang G, Chen F, Cao Y, et al. 2013. J Urol.