Recombinant Human NRG1-β2 #abs00925

abs00925-10ug

1-2 Weeks

138

abs00925-50ug

1-2 Weeks

344

abs00925-1mg

1-2 Weeks

1945

Source

Escherichia coli.

Format

The solution in PBS, pH 7.4 was filtered through a 0.2 μm filter and then lyophilized. To create a similar but unique content, we could say: The pH 7.4 PBS solution underwent filtration through a 0.2 μm filter before being subjected to lyophilization.

Properties

aa_sequence

NGGECFMVKDLSNPSR SHLVKCAEKE TYLCKCPNEF QNYVMASFYKAEELY TGDRCQ.

Concentration

> 96 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The Recombinant Human NRG1-β2 has been determined to have less than 1 EU/μg, as per the LAL method. An equivalent content can be formed by rearranging the above information while retaining its original meaning.

Activity

The standard and the ED have comparable biological activities, suggesting that the ED is fully active. Please rearrange the content to create a highly similar statement while maintaining the original information.₅₀The specific activity of the cell proliferation assay, utilizing serum free human MCF-7 cells, showcases that the determined concentration is less than 50 ng/ml. This indicates that the activity is quite potent, with a specific activity surpassing 2.0 × 10. These findings highlight the potential efficacy of this particular assay and the importance of using effective testing methods in research.⁴ IU/mg.

Reconstitution

For optimal results, we advise briefly centrifuging the vial before opening it to ensure that the contents settle at the bottom. To reconstitute, add sterile distilled water or an aqueous buffer containing 0.1% BSA until the concentration reaches 0.1-1.0 mg/mL. It is recommended to divide the stock solutions into smaller aliquots for practical use and store them at or below -20 °C. Subsequent dilutions can be performed using suitable buffered solutions.

Stability & Storage

This lyophilized product can be stored at a temperature range of 2-8 °C, however, it is recommended to keep it at -20 °C for long-term storage, with desiccation being preferred. When reconstituted, the product remains stable for a period of one week if stored at 2-8°C. To maximize its stability, divide the reconstituted preparation into smaller, usable portions and store them at a temperature range of -20 °C to -70 °C. It is important to avoid repeated freeze/thaw cycles to prevent any potential loss of stability.

Target

Accession

Q02297

Gene IDs

3084

References

References

1. Shamir A, Kwon OB, Karavanova I, et al. 2012. J Neurosci, 32: 2988-97.

2. Malek RL, Toman RE, Edsall LC, et al. 2001. J Biol Chem, 276: 5692-9.

3. Zhang Z, Prentiss L, Heitzman D, et al. 2006. J Neurosci Res, 84: 1-12.

4. Law AJ, Shannon Weickert C, Hyde TM, et al. 2004. Neuroscience, 127: 125-36.