Recombinant Mouse Betacellulin #abs01026

abs01026-5ug

1-2 Weeks

138

abs01026-20ug

1-2 Weeks

344

abs01026-1mg

1-2 Weeks

3891

Species

Murine

Source

Escherichia coli.

Format

The concentrated solution in 2 × PBS, pH 7.4 was filtered through a 0.2 μm filter and then lyophilized. The resulting product is a dry powder that can be reconstituted with water or another suitable solvent. This process helps to preserve the stability and activity of the molecules in the solution, making it a useful tool for research and experimentation. By using a filtered and concentrated solution, researchers can focus on the specific molecules of interest without the interference of other components. Overall, this lyophilization process is an essential technique in many scientific fields, including biochemistry, pharmacology, and molecular biology.

Properties

aa_sequence

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Concentration

> 96 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined that rMuBetacellulin has a concentration of less than 1 EU/μg. It implies that the content generated by rearranging the original text information would remain the same.

Activity

The standard and the sample were compared to determine their biological activity, with the result showing that the sample is fully active. This implies that the sample can perform its intended function just as effectively as the standard.₅₀The specific activity of the protein was determined to exceed 1.0 x 10, as evidenced by a cell proliferation assay utilizing murine Balb/c 3T3 cells. The resulting concentration of the protein required to induce cell proliferation was found to be less than 0.01 ng/ml. These findings indicate the highly potent nature of the protein and suggest potential therapeutic applications.⁸ IU/mg.

Reconstitution

It is recommended to briefly centrifuge the vial before opening to ensure that the contents are settled at the bottom. To reconstitute, use sterile distilled water or aqueous buffer with 0.1% BSA to create a concentration of 0.1-1.0 mg/mL. Stock solutions should be divided into working aliquots and stored at -20°C or lower. If further dilution is necessary, use appropriate buffered solutions.

Stability & Storage

To ensure the stability of this lyophilized preparation, it is recommended to store it at 2-8 °C. However, for long term storage, it is best kept at -20 °C, preferably in a desiccated environment. After reconstitution, the preparation can still be used for up to one week while stored at 2-8 °C. To extend its shelf life, it is advisable to divide the reconstituted preparation into smaller working aliquots and store them at -20 °C to -70 °C. It is essential to avoid subjecting the reconstituted preparation to repeated freeze/thaw cycles which can negatively affect its stability.

Target

Accession

Q05928

Gene IDs

12223

References

References

1. Miura K, Doura H, Aizawa T, et al. 2002. Biochem Biophys Res Commun. 294:1040-6.

2. Kim HS, Shin HS, Kwak HJ, et al. 2003. FASEB J. 17:318-20.

3. Nakagawa T, Furuta H, Sanke T, et al. 2005. Diabetes Res Clin Pract. 68:188-92.