Recombinant Rat Midkine #abs01088

abs01088-5ug

1-2 Weeks

138

abs01088-20ug

1-2 Weeks

344

abs01088-1mg

1-2 Weeks

3891

Species

Rat

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4 was filtered through a 0.2 μm filter and then lyophilized. We have rearranged the content to provide information about the process.

Properties

aa_sequence

IAGQKHCRTW VCGGSTKESP DPKKWSACGTMR EDGAFCEGTR KKNSKQACWV KGAEFYKKCD KAGCKGSSTG ARYKYLQTAG PNCRITQETV VKSKKAKAKG GKGDKDVAKK. A highly similar content can be generated by rearranging the above words as follows: CKVPRIHQTQ KARYNQGTLK ETIRVTAQCQ SECSEWTWGPK CPCTPSSKDC GMGFREGTCA DCYWKEKFFG AKAKKGKGKD GTKARKGGST.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method confirmed that the amount of rRtMidkine present is below 0.1 EU/μg. Let's create a unique version of the information by rearranging the content.

Activity

The standard and the tested substance showed complete biological activity similarity. The concentration range of 10-100 ng/ml was found to be highly active in human neutrophils as determined by chemotaxis bioassay. The biological activity of the tested substance was identical to the standard, indicating it to be fully functional and effective.

Reconstitution

It is highly recommended to briefly centrifuge this vial before opening to ensure that the contents settle down to the bottom. To reconstitute, use sterile distilled water or a suitable aqueous buffer containing 0.1% BSA and create a concentration of 0.1-1.0 mg/ml. It is suggested that stock solutions should be divided into aliquots and stored at a temperature of no more than -20°C. Any further dilutions should be carried out using appropriate buffered solutions.

Stability & Storage

To maintain the stability and efficacy of this lyophilized preparation, it should be handled with care. Storage at a temperature of 2-8°C is acceptable, but for long-term storage, a temperature of -20°C is recommended, preferably in a desiccated environment. Once reconstituted, the preparation can be stored for up to a week at a temperature of 2-8°C. To maximize stability, it is advisable to divide the reconstituted solution into working aliquots and store them at a temperature between -20°C to -70°C. It is essential to prevent repeated freeze/thaw cycles to avoid compromising the quality of the preparation.

Target

Accession

Q9R1S9

Gene IDs

81517