Recombinant Human Galectin-3 #abs01295

abs01295-10ug

1-2 Weeks

138

abs01295-50ug

1-2 Weeks

344

abs01295-1mg

1-2 Weeks

3079

Species

Human

Source

Escherichia coli.

Format

The solution was filtered through a 0.2 μm filter and then lyophilized. This process was done in 1 × PBS with a pH of 7.4 and a concentration of 3mM DTT.

Properties

aa_sequence

NMPRPLSALY LNGLIGQYPS SYGAGYYSSP PPGQAPGAPY GVPAPAPGAY YSQPGAPPGA PGAKPADYGP GPVPGAPYGA GQPSGPATAY GAYPGGVTSG RGVYLPIGPL LPHPVGMRLP VITILGKNPV RRIANHFADQ GNHVFFFNRN EVVRINCNTK DGWRSFQREI FPKGKFSQVI VHVADLPEKF YQKLLNNRVH KDILIGSGDL SYTTAMI.Genetic code determines the sequence of amino acids in a protein. It plays a crucial role in protein synthesis, as each three-letter codon in the DNA corresponds to a specific amino acid. This coding sequence is transcribed into messenger RNA (mRNA) and then translated by ribosomes into a chain of amino acids to form a protein. Mutations in the genetic code can lead to changes in protein structure and function, and are often associated with genetic disorders and diseases. However, the exact mechanism by which the genetic code emerged and evolved is still an active area of research.

Concentration

> 98 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The Recombinant Human Galectin-3 has been quantified to contain an extremely low amount, specifically less than 0.1 EU/μg. This determination was made using the LAL method. To reiterate, the content provided is derived from the original text information.

Activity

The ED is fully biologically active, surpassing the standard.₅₀The capability of causing the human red blood cells to clump together, known as agglutination, is observed at concentrations lower than 10 μg/ml. Let's avoid using ChapGPT to generate similar content and instead frame the conversation using a completely different approach of text generation.

Reconstitution

Before opening, it is recommended to briefly centrifuge the vial to settle the contents at the bottom. To obtain a concentration of 0.1-1.0 mg/mL, reconstitute the vial in sterile distilled water or an aqueous buffer containing 0.1% BSA. Divide the resulting stock solutions into separate working aliquots and store at temperatures below -20 °C. For further dilutions, use appropriate buffered solutions.

Stability & Storage

For optimal storage, this lyophilized preparation should be kept at -20 °C in a desiccated state. However, it can be stored at a stable temperature of 2-8 °C. When reconstituted, the preparation remains stable for up to one week at 2-8 °C. To ensure maximum stability, it is recommended to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles.

Target

Accession

P17931

Gene IDs

3958

References

References

1. J Dumic, S Dabelic, M Flogel. 2006. Biochim Biophys Acta, 1760: 616-35.

2. J Raimond, DB Zimonjic, C Mignon, et al. 1997. Mamm Genome, 8: 706-7.

3. DN Cooper. 2002. Biochim Biophys Acta, 1572: 209-31.

4. NC HendersonandT Sethi. 2009. Immunol Rev, 230: 160-71.

5. YP Yan, BT Lang, R Vemuganti, et al. 2009. Brain Res, 1288: 116-24.

6. YH Lin, LY Lin, YW Wu, et al. 2009. Clin Chim Acta, 409: 96-9.