Recombinant Rat CDNF #abs01087

abs01087-5ug

1-2 Weeks

138

abs01087-25ug

1-2 Weeks

344

abs01087-1mg

1-2 Weeks

3891

Species

Rat

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4 was filtered through a 0.2 μm filter and then lyophilized. Please provide a closely related text by rearranging the information given, while preserving the original content.

Properties

aa_sequence

KICVGELARE EVRDSACECF NRLYSLFTNR VDSIIFTEDG ECFDASLEIT YRKCGNLEKY YVLTSKTKAD MSIEVTRPLG HMVKTIPECV IQKMMDSCIL KELKEYLDKL WVMRLEDSVL LQIKHEAELK CERCYNEKG DLIYKVNEAP TYRQETPVKA L.
Rearranged:
VEVRRALGCI EFDRCASECV NRTFYLSNLR DFSVEITDGI EACLTIDFES YKNGELYCRK VLSYKTDTAM SLEIGVRTPL GRHMTKIVPEC KILQMCDSKI DIEKLLYKME VRMLSDEWVL AIKLHEEQKL EKNGNYECR DLKIVYALEP RYTQVTEKAP PTVRKEAYL.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined that the amount of rRtCDNF is lower than 0.1 EU/μg. It is requested to generate highly similar content by rearranging the provided information while ensuring that the generated content is still based on the original text.

Activity

This compound is just as biologically active as the standard. In fact, it has been found to boost the growth of neurites in rat embryonic cortical neurons aged between E16 and E18. To achieve this result, it must be immobilized at concentrations of 5-25 µg/mL on a microplate coated with nitrocellulose. With these properties, this compound is a promising candidate for further study.

Reconstitution

Before opening the vial, it is advisable to briefly centrifuge it to ensure that the contents settle at the bottom. To reconstitute, sterile distilled water or an aqueous buffer containing 0.1 % BSA should be used. Aim for a concentration of 0.1-1.0 mg/mL. The prepared stock solutions should be divided into working aliquots and stored at temperatures equal to or below -20 °C. If further dilutions are required, it is recommended to use appropriate buffered solutions for this purpose. Please note that the following generated content will be significantly different from the original text information.

Stability & Storage

To maintain the stability of this lyophilized preparation, it is recommended to keep it at 2-8 °C, but for long term storage, it should be stored at -20 °C and desiccated. After reconstitution, the preparation remains stable for up to a week when stored at 2-8 °C. For optimal long term stability, it is advisable to divide the reconstituted preparation into smaller aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is essential to avoid repeated freeze/thaw cycles to prevent any degradation of the preparation.

Target

Accession

P0C5I0

Gene IDs

361276