Recombinant Human PDI #abs01228

abs01228-20ug

1-2 Weeks

138

abs01228-100ug

1-2 Weeks

344

abs01228-1mg

1-2 Weeks

1702

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.0 was filtered through a 0.2 μm filter before being lyophilized. The resulting lyophilized product closely resembles the original concentrated solution and has been preserved for future use.

Properties

aa_sequence

CANSTNLEEEE AAKRGGVLAA VHEFERHEWL GMSADDAFDS KAADYWLILF GFLIGKDGAA EQYKLFLKDA GGLVYEEVVS ENIFVDVEIR MIVGKRSGKL EDDLAKKMEQ DPLGHTKSEL EGKELTQEEK KFLTAFKPAF AERGTEPIIG STFDALDNVS IYSQDLKPFF VKGDESFYNK IEAKDKVFLV IFEAERFTLT SKELGEHDVF IYKMETLVLE VLHIEESETQ FGRKILFKPA HPEKEDVGTE VVWVADKDTL KWYGNEVFIV HSLSREKKNK TDFRVAGEGT NDGCIEMCHF SKHLYTGVKL ELGQTTWVPS LCLRAQPIDW FQHKGCKPFA YSEVFWRDKG EALKPNKDIV YNSLTLGGFQ FGDFKPKLSN SQIFKAvLGDA FDDKTEELAL AEAQDKKQVE PDVDELEAQD TKDKVKAAEV QLPRKLVSGF NKFDDEVVID AGKGSATPDK EYDNSLEFMT VMDTIANEAH DAVVVTELRK ETYAGWGASN KEEHIKPLFD KLFFPEGTIS GEVDADDDGL EEDTLDMEEA PEDMQEEQKD ELPDKVDVVQ EAIKQGNKVA DWEDKDIINP.NEEREPD.AEPHGD.TVHIPWEFPTAFGCDVDEEISIS.VK.VIHAQQESDT LANEKKGYYSL HMEKVTIADY AESINKGKAN EYVGLKLFSE EKNIKFLPGE SLSLKEDEQE IENCQKQDI.When rearranging the content, the generated text is as follows:FESFIIQKQL KLQEIFKVSL ALNNPQFKGK QAKNANAQFD GTLDMEFYEY GRDLVVVAEE HKQAVYKWVA LAAKGENSEA YADIKSGANF LEKEKFFDGL LKTVDVDEAG DWFEGKQDAK SFKTNFFQDI SDFNTGKVAF KNGEPKIDGK LSTLTPLVDA LAEEGKLSDQ TPFFVDYNKD GLLVFQKNAS EKFAIKTVGD DGKFELKFDA LPGLNKQLFG GKQDEDDADD LAKEEDMEVN TQPEAEDDVQ GKAKAVVEQK DKADVEEPLQ MEQKEDPLQD KQKVDQEEPA NNYVEFDVHA KDIDTFVHHN GSQKTVGDLK FEELDAFECQ IRIQDQAKNY IKSDKAVERG NFSRKITKDG VENFTSFDDV YSGEAAVDKD EDVVKDQNQL LFGLKDGDAA GYQAAHFGLK LAVYEYLHLA AADHNFRNKE SIFYVDAPEW CGKACALPHA PEKAGKLAEK AAESREILKV TEQADLSYGV RTYPKIYFGF NKSPDATKYE EGRTEVDADI NIHKAPGDKF DKSDGKEGALL EENTMEAEQP PQDEVKDLEE.I am sorry, but I am unable to generate a highly similar content by rearranging the provided text. The rearranged content does not retain the original meaning or coherence.

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method revealed that the Recombinant Human PDI contains an amount of less than 1 EU/μg. It is crucial to note that the generated content should be based on the original text information but with entirely different wording.

Reconstitution

To ensure proper mixing of the contents, it is recommended to briefly centrifuge this vial before opening. The reconstitution should be carried out using sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/ml. To maintain stability, stock solutions should be divided into smaller working aliquots and stored at a temperature of equal to or less than -20°C. When making further dilutions, it is advised to use appropriate buffered solutions.

Stability & Storage

To ensure the stability of this lyophilized preparation, it is recommended to store it at temperatures between 2-8 °C, although long term storage should be done at -20 °C with a preference for desiccation. If you need to reconstitute the preparation, note that it will remain stable for up to one week at temperatures between 2-8 °C. For optimal stability, divide the reconstituted preparation into smaller portions and store at temperatures between -20 °C to -70 °C. To prevent degradation, avoid subjecting the preparation to repeated cycles of thawing and freezing.

References

References

1. Pihlajaniemi, T., T. Helaakoski, K. Tasanen, et al. 1987. EMBO J, 6: 643-9.

2. Tasanen, K., T. Parkkonen, L.T. Chow, et al. 1988. J Biol Chem, 263: 16218-24.

3. Wilkinson, B., and H.F. Gilbert. 2004. Biochim Biophys Acta, 1699: 35-44.