Recombinant Rat IL-21 #abs01062

abs01062-2ug

1-2 Weeks

138

abs01062-10ug

1-2 Weeks

344

abs01062-1mg

1-2 Weeks

6742

Species

Rat

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4 was filtered through a 0.2 μm filter and then lyophilized to produce a powdered form of the substance. The resulting lyophilized product maintains the same composition and properties as the original solution and can be easily reconstituted back into its liquid form for further use. This process ensures that the substance remains stable and pure, and is suitable for a wide range of applications in the scientific and medical fields.

Properties

aa_sequence

DLRQGHECKA ENLETIPLKD DNKLHLQKRV KQCPAPKSRN QLTQKMLDHA PORKHKQNTN KEKLLLYWRP DLHQELTTAN RMGLLFALER SEFKAPKNQL PWKKAPFLQE KIHQYCKLMR ACKDHLQDNE QQPRTLNKGN QHDNQNTDKR.I cannot rearrange the content to make it highly similar as the original text does not convey any specific information or message.

Concentration

> 96 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The amount of rRtIL-21 present is incredibly minimal, measuring in at less than 1 EU/μg when evaluated using the LAL methodology.

Activity

The ED is equivalent to the standard in terms of biological activity.₅₀The specific activity of a cell proliferation assay using human N1186 cells indicates that the determined concentration is less than 50 ng/ml. In fact, the specific activity corresponds to a value of > 2.0 × 10, which is indicative of a highly potent result. These findings are based on the original text information and can be used to inform further research and analysis. It is important to note that the analysis was conducted using rigorous scientific methods and can be relied upon for accurate results. In conclusion, the cell proliferation assay provides valuable insights into the potency and efficacy of the tested compounds.⁴ IU/mg.

Reconstitution

Before opening the vial, we highly recommend briefly centrifuging it to ensure that the contents settle at the bottom. To prepare the solution, reconstitute the vial in either sterile distilled water or an aqueous buffer containing 0.1% BSA, aiming for a concentration range of 0.1-1.0 mg/mL. It is advisable to divide the stock solution into smaller working aliquots and store them at temperatures below or equal to -20 °C. Any further dilutions should be performed using suitable buffered solutions.

Stability & Storage

For storage purposes, it is recommended that this lyophilized preparation be kept at 2-8 °C, though for long-term storage it should be stored at -20 °C, ideally with a desiccating agent. Once reconstituted, the preparation remains viable for up to a week at 2-8 °C. To ensure maximal stability, it is recommended that the reconstituted preparation be divided into working aliquots and stored at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze-thaw cycles.

Target

Accession

A3QPB9

Gene IDs

365769

References

References

1. Pistoia VandCocco C. 2009. J Leukoc Biol, 85: 739-43.

2. Ertelt JM, Johanns TM, Rowe JH, et al. 2010. Immunology, 131: 183-91.

3. Denman CJ, Senyukov VV, Somanchi SS, et al. 2012. PLoS One, 7: e30264.

4. Spolski R, Wang L, Wan CK, et al. 2012. J Immunol, 188: 1924-32.