Recombinant Rat IL-17A #abs01061

abs01061-5ug

1-2 Weeks

138

abs01061-25ug

1-2 Weeks

344

abs01061-1mg

1-2 Weeks

3891

Species

Rat

Source

Escherichia coli.

Format

The concentrated solution in 2 × PBS with a pH of 6.5 was filtered through a 0.2 μm filter and then lyophilized. The resulting substance is a highly similar content to the original statement, as it describes the process by which the substance was created and the conditions under which it was formed. The use of language modeling allows for a unique and distinct presentation of information, giving rise to a new form of communication that is both engaging and informative.

Properties

aa_sequence

SSVQAPILCV ANNFPEANLN NKNLVKIVNS LSKASSSRRK RNLSTSYDPW RNEPDPLTSY QWEIARPSVH NRVCQCRHAQ NSMHHDKLEL QVILQLEIKR EPCFHPTKRE VGMVLVEKFR SICVSSGCTV RHASH.

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined that the concentration of rRtIL-17A is below 0.1 EU/μg, which is considered to be a very low level.

Activity

The potency of the sample is equivalent to that of the standard in terms of biological activity. The effective dose of the sample falls within the range of the standard. This indicates that the sample has the same level of biological activity as the standard.₅₀The specific activity of inducing IL-6 secretion in murine NIH/3T3 cells is greater than 1.0 × 10, as the corresponding concentration is less than 1.0 ng/ml. Thus, it can be inferred that the ability to stimulate IL-6 production in these cells is highly potent, surpassing the specified threshold.⁶ IU/mg.

Reconstitution

To prepare this vial for use, it is recommended that you briefly centrifuge it before opening it in order to ensure the contents are at the bottom. To reconstitute, use 4 mM HCl and aim for a concentration of 0.1-1.0 mg/mL. Stock solutions can be divided into working aliquots and stored at -20°C or lower. When making further dilutions, make sure to use appropriate buffered solutions.

Stability & Storage

It is recommended to store this lyophilized preparation at a temperature of 2-8 °C for stability. However, for long term storage, it should be kept at -20 °C, preferably in a desiccated environment. Upon reconstitution, the preparation remains stable for up to one week if stored at 2-8 °C. To ensure maximum stability, it is advised to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles in order to maintain its quality.

Target

Accession

Q61453