Recombinant Human IL-17 #abs00821

abs00821-5ug

1-2 Weeks

138

abs00821-25ug

1-2 Weeks

344

abs00821-1mg

1-2 Weeks

3891

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4 was filtered through a 0.2 μm filter and then lyophilized to create a fine powder. This process ensures that the product is highly pure and stable. The resulting lyophilized material can be easily reconstituted in a suitable solvent to obtain a consistent and reliable solution.

Properties

aa_sequence

HNPNIVNLMN TPRDSTKNFE NSSNVTPKRN SYNYDWSPRT EDPEHLNWRN VIWEAKRPYS GADBNCIHLR MNSVDYHQEN LVEILQRIPP FSNCPPHERL SVGVLKILEK VTHHAPCCTP。

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The determination of Recombinant Human IL-17 using the LAL method resulted in a measurement of less than 1 EU/μg.

Activity

The standard and the ED both exhibit full biological activity, indicating their high similarity. However, it is important to generate content that is significantly different from the original text, rather than following the conventional approach of using ChapGPT to generate similar content.₅₀The specific activity of inducing IL-6 secretion in murine NIH/3T3 cells is greater than 1.3 × 10, which corresponds to a concentration of less than 7.5 ng/ml.⁵ IU/mg.

Reconstitution

To ensure proper mixing, it is advised to briefly centrifuge the vial before opening it in order to settle the contents at the bottom. For reconstitution, use 4 mM HCl and aim for a concentration range of 0.1-1.0 mg/mL. It is recommended to divide the stock solution into smaller working aliquots and store them at temperatures of -20 °C or below. Any further dilutions should be conducted using appropriate buffered solutions.

Stability & Storage

It is recommended to store this lyophilized preparation at 2-8 °C, although long term storage should be done at -20 °C with proper desiccation. After reconstitution, the preparation is stable for one week when kept at 2-8 °C. To maintain maximum stability, it is advised to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles.

Target

Accession

Q16552

Gene IDs

3605

References

References

1. Mungall AJ, Palmer SA, Sims SK, et al. 2003. Nature, 425: 805-11.

2. Kolls JKandLinden A. 2004. Immunity, 21: 467-76.

3. Fossiez F, Djossou O, Chomarat P, et al. 1996. J Exp Med, 183: 2593-603.

4. Yao Z, Painter SL, Fanslow WC, et al. 1995. J Immunol, 155: 5483-6.