Recombinant Human IL-1RA #abs00803

abs00803-20ug

1-2 Weeks

138

abs00803-100ug

1-2 Weeks

344

abs00803-1mg

1-2 Weeks

1425

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4 was filtered through a 0.2 μm filter and then lyophilized for preservation. A highly similar content can be generated by rephrasing the original text, keeping the information intact.

Properties

aa_sequence

IPLGAVLNQK KYMFRIWQVA GRKSSNRLNQ YVAGDQNPLE LEINKDVDVP IEPHGFFLAL HGSKMCLCKV DGERSTMQLK ENVDIATNSL EQRRKQDAFF ISDPTSGSSE FACSAWGCPW LCTEPADAMV SLNVTPDEMG VVMTKFYEQF EDKQRNIFAS.

Concentration

> 96 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method revealed Recombinant HumanIL-1RA contains less than 1 EU/μg. It is essential to generate a unique content while retaining the original information from the text.

Activity

The standard is significantly enhanced when compared to the original, rendering it fully biologically active. This ensures that the ED is effectively addressed.₅₀The specific activity of < 2.5 × 10 corresponds to a concentration of less than 40 ng/ml, which effectively inhibits the IL-1α-dependent proliferation of murine D10S cells.⁴50 pg/ml IL-1α，IU/mg。，。

Reconstitution

To ensure the proper distribution of the contents, it is advised to centrifuge the vial briefly before opening. For reconstitution, use either sterile distilled water or an aqueous buffer with 0.1% BSA at a concentration ranging from 0.1 to 1.0 mg/mL. It is recommended to divide the stock solutions into smaller aliquots for practical use and store them at temperatures below or equal to -20 °C. When making further dilutions, use appropriate buffered solutions.

Stability & Storage

To keep this lyophilized preparation stable, it is recommended to store it at 2-8 °C. However, for long term storage, it should be kept at -20 °C, and preferably desiccated. Once reconstituted, the preparation remains stable for up to one week when stored at 2-8 °C. For maximum stability, it is advisable to divide the reconstituted preparation into working aliquots and store them at temperatures between -20 °C to -70 °C. It is crucial to avoid subjecting the reconstituted preparation to repeated freeze/thaw cycles to prevent any potential damage.

Target

Accession

P18510

Gene IDs

3557

References

References

1. Dinarello, C.A. 1994. FASEB J, 8: 1314-25.

2. Perrier, S., F. Darakhshan, and E. Hajduch. 2006. FEBS Lett, 580: 6289-94.

3. Dinarello, C.A. 1994. FASEB J, 8: 1314-25.