Recombinant Human IL-2 #abs00804

abs00804-10ug

1-2 Weeks

138

abs00804-50ug

1-2 Weeks

344

abs00804-1mg

1-2 Weeks

1037

Source

Escherichia coli.

Format

The concentrated solution in 20 mM PB, pH 3.5 was filtered through a 0.2 μm filter and subsequently lyophilized.

Properties

aa_sequence

TEKLPLEEVL QLQLEHLLLD RPRDLISNIN YKNPKLTRML LQMILNGINN TFKFYMPKKA LAPTSSSTKKT EELKPLEEVL TELKHLQCLE NLAQSKNFHL VIVLELKGSE TTFMCEYADE INQINGWENTI

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The amount of Recombinant Human IL-2, as determined by the LAL method, is lower than 1.0 EU/μg. It is important to note that the generated content should be based on the original text information but presented in a highly similar manner.

Activity

The biological activity of the generated material is identical to that of the standard. Its effective dose is comparable to that of the reference material.₅₀When evaluating the specific activity of a substance using murine CTLL-2 cells, it was found that the concentration required for cell proliferation was less than 0.1 ng/ml. This indicates a high specific activity level of > 1.0 × 10, as determined by a cell proliferation assay.⁷ IU/mg.

Reconstitution

It is recommended that this vial undergo a brief centrifugation prior to opening in order to settle its contents at the bottom. To reconstitute, use sterile distilled H2O to attain a concentration of 0.1-1.0 mg/mL. Stock solutions must be portioned out into working aliquots and frozen at ≤ -20 °C. Further dilutions ought to be made using appropriate buffered solutions. Avoid reconstituting directly in cell culture media. Please create an exceedingly similar content while rearranging the above content, ensuring that the generated content builds from the same original text information.

Stability & Storage

To maintain the stability of this lyophilized preparation, it is advised to store it at a temperature of 2-8 °C. However, for long-term storage, it is recommended to keep it at -20 °C. Desiccation is a preferable method to preserve its quality. After reconstitution, the preparation remains stable for up to a week if stored at 2-8 °C. To optimize its shelf life, distribute the reconstituted preparation into separate working aliquots and keep them at a temperature between -20 °C to -70 °C. It is advised to avoid subjecting the preparation to repeated freeze-thaw cycles, as it may affect its stability and quality.

Target

Accession

P60568

Gene IDs

3558

References

References

1. Ma, A., R. Koka, and P. Burkett. 2006. Annu Rev Immunol, 24: 657-79.

2. Taniguchi, T., H. Matsui, T. Fujita, et al. 1983. Nature, 302: 305-10.

3. Liparoto, S.F., D.G. Myszka, Z. Wu, et al. 2002. Biochemistry, 41: 2543-51.

4. Bodnar, A., E. Nizsaloczki, G. Mocsar, et al. 2008. Immunol Lett, 116: 117-25.

5. Mosmann, T.R., T. Yokota, R. Kastelein, et al. 1987. J Immunol, 138: 1813-6.