Recombinant Human IL-3 #abs00805

abs00805-2ug

1-2 Weeks

138

abs00805-10ug

1-2 Weeks

344

abs00805-1mg

1-2 Weeks

3891

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4 was filtered through a 0.2 μm filter and then lyophilized to form a dry substance. To create content that closely matches the original text, the wording can be rearranged as follows: The solution was concentrated and filtered through a 0.2 μm filter before being dissolved in PBS, pH 7.4. The resulting solution was then lyophilized to form a powdered substance.

Properties

aa_sequence

AALDNICKSV NVFQPTLMTS LPEQNLIHID QNEIPDLTLP LDMFNNPLLL EMILDDQNGN LERNLPNRFA LQNLSKVARN LKNLISESAI AATPALLPCL KIHRPHPATA RRKRFNEWDG NETLKTYLFL LSLTLTQQAQ FISL.

Concentration

> 96 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined that the Recombinant HumanIL-3 has a potency of less than 1 EU/μg. In order to present the information in a highly similar manner, the content can be rearranged as follows: According to the LAL method, the determination of Recombinant HumanIL-3 potency reveals that it is less than 1 EU/μg.

Activity

The active biological properties are equivalent to the standard version. This means that it possesses the same biological activity as the original substance, ensuring that it is a highly effective product.₅₀The specific activity of a certain protein was found to be greater than 1.0 × 10 when examined using human TF-1 cells in a cell proliferation assay. This was achieved by using a concentration of the protein that was less than 0.1 ng/ml. These results highlight the promising potential of this protein and its potential use in various applications.⁷ IU/mg.

Reconstitution

Prior to opening, it is recommended to briefly centrifuge this vial in order to settle the contents at the bottom. To achieve a concentration of 0.1-1.0 mg/mL, reconstitute the vial in sterile distilled water or an aqueous buffer containing 0.1% BSA. It is advisable to divide the stock solutions into smaller working aliquots and store them at a temperature of ≤ -20 °C. For further dilutions, use appropriate buffered solutions.

Stability & Storage

It is important to store this lyophilized preparation properly to ensure its stability. It can be kept at 2-8°C, but for long term storage, it is best to store it at -20°C and to keep it desiccated. Once reconstituted, it can stay stable for up to a week when stored at 2-8°C. However, for maximum stability, it is recommended to divide the reconstituted preparation into smaller working aliquots and store them at -20°C to -70°C. It is best to avoid repeatedly freezing and thawing the preparation as this can affect its stability.

Target

Accession

P08700

Gene IDs

3562

References

References

1. Yang YC, Ciarletta AB, Temple PA, et al. 1986. Cell. 47:3-10.

2. Otsuka T, Miyajima A, Brown N, et al. 1988. J Immunol. 140:2288-95.

3. Dorssers L, Burger H, Bot F, et al. 1987. Gene. 55:115-24.

4. Feng Y, Klein BK, McWherter CA. 1996. J Mol Biol. 259:524-41.