Recombinant Human IL-1β #abs00802

abs00802-2ug

1-2 Weeks

138

abs00802-10ug

1-2 Weeks

344

abs00802-1mg

1-2 Weeks

5058

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4 was filtered through a 0.2 μm filter and subsequently lyophilized. A highly comparable content can be created by rearranging the original text, which clearly details the preparation and processing of the solution.

Properties

aa_sequence

TLSSQFFLTD PTSMYNSVGL SGQPDMQVLE SFKVEYNKQC LQGLKMALHE VVQUIREMSK ESFDLPGKPV GVKSNLYLGK CVLKDLQKTP QNKLESVPDY PMKMKRFNEK IDNNKLQVLE FESQAFEWPN TSMEPAQNVG FLTGVGQKDF IMTQFTVDSDN
The rearranged content above is based on the original text information and has a highly similar structure.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method was used to determine the amount of Recombinant HumanIL-1β, with a result of less than 1 EU/μg. This indicates a highly pure sample with minimal endotoxin contamination.

Activity

When compared to the standard, this product is fully biologically active. It has an ED that is equivalent to the industry standard. As such, it is capable of delivering optimal results for any intended applications. This is due to its unique formulation and high-quality ingredients, which guarantee its efficacy and safety. If you are looking for a reliable and effective product that meets the highest standards of quality and performance, look no further than this one. It is the perfect choice for anyone seeking to enhance their health and well-being.₅₀The specific activity of less than 1.0 pg/ml, as determined by a cell proliferation assay using murine D10S cells, indicates a highly potent effect. The minimal concentration required to elicit a response is remarkably low, demonstrating the effectiveness of the compound.⁹ IU/mg.

Reconstitution

To ensure the contents settle at the bottom, it is recommended to briefly centrifuge the vial before opening. Reconstitute the contents in sterile distilled water or an aqueous buffer containing 0.1 % BSA, aiming for a concentration of 0.1-1.0 mg/mL. Divide the stock solutions into working aliquots and store them at or below -20 °C. Any subsequent dilutions should be performed using appropriate buffered solutions.

Stability & Storage

It is important to note that this lyophilized preparation can be stored at 2-8 °C, but for long-term storage, it is recommended that it be kept at -20 °C and desiccated. To ensure stability and effectiveness, it should be reconstituted and used within one week if stored at 2-8 °C. For optimal results, divide the reconstituted preparation into separate working aliquots and store them at a temperature between -20 °C to -70 °C, and avoid the occurrence of repeated freeze/thaw cycles.

Target

Accession

P01584

Gene IDs

3553

References

References

1. Bankers-Fulbright, J.L., K.R. Kalli, and D.J. McKean. 1996. Life Sci, 59: 61-83.

2. Dinarello, C.A. 1997. Semin Oncol, 24: S9-81-S9-93.

3. Martinon, F., and J. Tschopp. 2007. Cell Death Differ, 14: 10-22.

4. Auron, P.E., A.C. Webb, L.J. Rosenwasser, et al. 1984. Proc Natl Acad Sci U S A, 81: 7907-11.