Recombinant Rat IL-3β #abs01052

abs01052-5ug

1-2 Weeks

138

abs01052-20ug

1-2 Weeks

344

abs01052-1mg

1-2 Weeks

3891

Species

Rat

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4 was filtered through a 0.2 μm filter and then lyophilized.

Properties

aa_sequence

DPTSVDEKTK SRQNKKDVDD LKLRSPFYFL QPHKDSVAPE SMSKKIQQLP LSNSRGIKHA SLEEVRDKLK DTTNISISDV EQYAAFTLVC PGLPNKFKRQ KMIKDSYDLS PNIVKSLSGF QDLNNDPRKT TSPRDNNSID LTVPMFDPVQ PDSKSQRVFL ISDDKKVINR KQFVHEQNVK ELSPIELSDR.

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The amount of rRtIL-3β detected using the LAL method is below 1 EU/μg. To create similar content while rearranging the original text, you could write:
According to the LAL method, the determined level of rRtIL-3β is less than 1 EU/μg.

Activity

The standard and the tested sample were found to be equally biologically active. The ED was not significantly different between them.₅₀The specific activity of a protein can be determined by measuring the cell proliferation assay using murine MC-9 cells. The protein's activity is considered high when its concentration is less than 10 ng/ml, which is equivalent to a specific activity greater than 1.0 × 10. To assess the activity of the protein, it is important to use a reliable method that provides accurate results. By performing such tests, researchers can better understand the functionality of proteins and their potential applications in various fields.⁵ IU/mg.

Reconstitution

To ensure proper handling of this vial, we advise a brief centrifugation prior to opening to ensure the contents settle at the bottom. For reconstitution, it is recommended to use sterile distilled water or an aqueous buffer containing 0.1% BSA, aiming for a concentration range of 0.1-1.0 mg/mL. When preparing stock solutions, it is important to divide them into smaller working aliquots and store them at temperatures at or below -20 °C. Any further dilutions should be carried out with the appropriate buffered solutions.

Stability & Storage

To maintain the stability of this lyophilized product, it is recommended to store it at 2-8 °C, although for long-term storage, it should be kept at -20 °C and preferably in a desiccated state. Once reconstituted, the preparation can be kept for up to one week at 2-8 °C. For optimal stability, we suggest dividing the reconstituted product into smaller working aliquots and freezing at -20 °C to -70 °C. It is important to avoid any repeated freeze/thaw cycles to prevent any degradation of the product.

Target

Accession

P97688

Gene IDs

24495

References

References

1. Yang YC, Ciarletta AB, Temple PA, et al. 1986. Cell. 47:3-10.

2. Otsuka T, Miyajima A, Brown N, et al. 1988. J Immunol. 140:2288-95.

3. Cohen DR, Hapel AJ, Young IG. 1986. Nucleic Acids Res. 14:3641-58.

4. Esandi MC, van Someren GD, Bout A, et al. 1998. Gene Ther. 5:778-88.

5. Dorssers L, Burger H, Bot F, et al. 1987. Gene. 55:115-24.