Recombinant Rat IL-4 #abs01053

abs01053-5ug

1-2 Weeks

138

abs01053-20ug

1-2 Weeks

344

abs01053-1mg

1-2 Weeks

4214

Species

Rat

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4, 5% trehalose was filtered through a 0.2 μm filter and then lyophilized. Let's discuss the process of lyophilization and its significance in preserving the integrity of the solution.
Lyophilization, also known as freeze-drying, is a process used to remove water from a solution without causing damage or compromising its structure. It involves freezing the solution and then subjecting it to a vacuum, allowing the frozen water to directly sublimate from a solid to a gas without going through the liquid phase. This results in the removal of water from the solution while leaving behind the solute, typically in a dry, powder-like form.
The use of a 0.2 μm filter prior to lyophilization ensures that any particulate matter or large molecules present in the solution are removed, resulting in a purified and concentrated solution. Filtering the solution through such a fine membrane prevents the passage of unwanted contaminants, ensuring the final lyophilized product is of high quality.
The addition of trehalose, a non-reducing disaccharide, to the concentrated solution serves as a cryoprotectant during the freezing process. Trehalose helps to protect the solution from harmful effects such as osmotic stress and ice crystal formation that can occur during freezing and lyophilization. By stabilizing the structure of proteins and other biomolecules, trehalose aids in maintaining their functionality and integrity throughout the lyophilization process.
Lyophilization of a solution in PBS, pH 7.4, with the inclusion of trehalose as a cryoprotectant, provides a method of long-term preservation. The resulting lyophilized product has an extended shelf life, as the absence of water prevents the degradation or microbial growth that can occur in a liquid form. Additionally, the reconstitution of the lyophilized product is relatively simple, allowing for easy preparation and use when needed.
It is important to note that the lyophilized product should be stored in a cool and dry environment to maintain its stability. Proper storage conditions ensure that the lyophilized product remains viable, retaining its original properties and functionality over an extended period of time.
In summary, the lyophilization process, preceded by filtration through a 0.2 μm filter and the inclusion of trehalose as a cryoprotectant, allows for the preservation of a concentrated solution in PBS, pH 7.4. The resulting lyophilized product has an extended shelf life and can be easily reconstituted when needed.

Properties

aa_sequence

TLKDFLESLK HGCNDSPLRE DVPPCLKNKS GVLGELRKLC RVLRKFYFPR IINTLNQVTE PDVLTATRNT SCTVNESTLT KGTPCTEMFV TENELICRAS RC NKCCICGDFX MFVSLRGKYLQS CTSMSRGVSGLNSLRS.

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined that the rRtIL-4 had less than 1 EU/μg. Can you provide me with content that conveys the same message but in a different way, using the available information?

Activity

The potency of the sample is equivalent to the standard in terms of biological activity. In other words, it has completely preserved the standard's effectiveness.₅₀According to an assay that measures cell proliferation in rat splenocytes, the determined concentration is below 1.0 ng/ml, indicating a specific activity greater than 1.0 × 10. Let's now generate a highly similar content by rearranging the original information.⁶ IU/mg.

Reconstitution

Before opening, we suggest briefly centrifuging the vial to settle the contents at the bottom. You can reconstitute the contents in sterile distilled water or an aqueous buffer that contains 0.1% BSA, aiming for a concentration of 0.1-1.0 mg/ml. To ensure stability, divide the stock solutions into working aliquots and store them at -20 °C or below. When making further dilutions, use appropriate buffered solutions.

Stability & Storage

The lyophilized preparation can be stored at 2-8 °C, but for long-term storage, it is recommended to keep it at -20 °C, preferably in a desiccated condition. Once reconstituted, the preparation remains stable for one week when stored at 2-8 °C. To ensure maximum stability, it is advised to divide the reconstituted preparation into smaller aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles.

Target

Accession

P20096

Gene IDs

287287

References

References

1. Sokol CL, Barton GM, Farr AG, et al. 2008. Nat Immunol. 9:310-8.

2. Hershey GK, Friedrich MF, Esswein LA, et al. 1997. N Engl J Med. 337:1720-5.

3. Yokota T, Otsuka T, Mosmann T, et al. 1986. Proc Natl Acad Sci U S A. 83:5894-8.

4. Eder A, Krafft-Czepa H, Krammer PH. 1988. Nucleic Acids Res. 16:772.

5. Walter MR, Cook WJ, Zhao BG, et al. 1992. J Biol Chem. 267:20371-6.