Recombinant Mouse IL-1β #abs00968

abs00968-2ug

1-2 Weeks

138

abs00968-10ug

1-2 Weeks

344

abs00968-1mg

1-2 Weeks

6742

Species

Murine

Source

Escherichia coli.

Format

The content above was lyophilized from a solution in PBS, pH 7.4, which was filtered through a 0.2 μm filter. The solution also contained 5 % trehalose and 0.02 % Tween-20.

Properties

aa_sequence

VQELSKLYGL YRQHLRIPRV QQKSLVLRDE LAKHELYDPS QNQQVNGNLI VQGEFSMSFV PAVIKPIDNS LYLSGKNKGK TPLDMKCVQE YPKQVDSELQ RFVMEKPKKE VKSFEKINVF NWPEFPSAEF QAHKSETQSI GGQNSNNGLF DTVSEMFTDI SSVPSTQXZY
The rearranged text is:
YRLRQHVPI GLVLDEQQKS LHALSDPYEK QQVNINGNQL VQGEFSMSFV PALKIPVDNK YLSGLKGKNL TPLDMKCVQM QYDSELQLES VMRFVKIKEN FPSAEFPNWY QAHKPISTSA GNNGQDSNLF TDISSVEMFT QXZYVPSTSQ

Concentration

> 96 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method has determined that the rMuIL-1β contains less than 1 EU/μg. This information can be used to ensure the quality of the product and its safety for use. Ensuring that the content of the product is consistent with the original specifications is important in order to maintain the efficacy of the product. By adhering to these standards, consumers and healthcare professionals can be confident in the safety and effectiveness of the product.

Activity

The effectiveness of the product is at par with the standard as it is fully biologically active. The ED of the product is not compromised and remains consistent with the benchmark.₅₀The specific activity of < 2 pg/ml, as determined by a cell proliferation assay using murine D10S cells, indicates a very low level. This confirms that the substance has a minimal effect on the cell growth rate. The results show that the substance is not a potent stimulator of cell proliferation, as it falls below the threshold of significance. Thus, its impact on the cells is negligible. It is important to note that a specific activity of > 5.0 × 10 was not observed, indicating a lack of any significant beneficial or detrimental effects on cell proliferation.⁸ IU/mg.

Reconstitution

To ensure the contents are settled at the bottom, we suggest a brief centrifugation before opening the vial. For reconstitution, use sterile distilled water or an aqueous buffer containing 0.1% BSA at a concentration of 0.1-1.0 mg/mL. It is recommended to divide the stock solutions into smaller working aliquots and store them at temperatures not exceeding -20°C. Any further dilutions should be carried out using suitable buffered solutions.

Stability & Storage

To ensure the stability of this lyophilized preparation, it is recommended that it be stored at a temperature range of 2-8 °C. However, for long-term storage, it should be kept at a temperature of -20 °C, preferably in a desiccated state. Once reconstituted, the preparation can be stored for up to one week at a temperature range of 2-8 °C. To maximize stability, it is advised to split the reconstituted preparation into working aliquots and store them at a temperature range of -20 °C to -70 °C. It is important to avoid repeated freeze/thaw cycles to maintain the quality of the preparation.

Target

Accession

P10749

Gene IDs

16176

References

References

1. Bankers-Fulbright, J.L., K.R. Kalli, and D.J. McKean. 1996. Life Sci, 59: 61-83.

2. Dinarello, C.A. 1997. Semin Oncol, 24: S9-81-S9-93.

3. Martinon, F., and J. Tschopp. 2007. Cell Death Differ, 14: 10-22.

4. Gray, P.W., D. Glaister, E. Chen, et al. 1986. J Immunol, 137: 3644-8.