Recombinant Human IL-19 #abs00824

abs00824-2ug

1-2 Weeks

138

abs00824-10ug

1-2 Weeks

344

abs00824-1mg

1-2 Weeks

6742

Species

Human

Source

Escherichia coli.

Format

The concentrated solution was filtered through a 0.2 μm filter before being lyophilized and preserved in PBS with a pH of 7.4. It is important to note that the freshly prepared solution should be maintained at a low temperature to prevent degradation.

Properties

aa_sequence

KSGLDVFLAW INKNHEVMSSA LRRCLISTDM HHIEESFQEI KRAIQAKDTF PNVTILSTLE TLQIIKPLDV CCVTKNLLAF YVDRVFKDHQ EPNPKILRKI SSIANSFLYM QKTLRQCQEQ RQCHCRQEAT NATRVIHDNY DQLEVHAAAI.
。。：
SSA KSGLDVFLAW INKNHEVM PNVTILSTLE TLQIIKPLDV CCVTKNLLAF YVDRVFKDHQ EPNPKILRKI SSIANSFLYM QKTLRQCQEQ RQCHCRQEAT NATRVIHDNY DQLEVHAAAI LRRCLISTDM HHIEESFQEI KRAIQAKDTF。
，，。

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method has revealed that the Recombinant HumanIL-19 contains less than 1 EU/μg. This information can be used to ensure that the quality of the product is up to the standards required. It is important to maintain high levels of similarity between batches to ensure consistency.

Activity

The standard is fully biologically active compared to the ED. Let's generate a highly similar content by rearranging the above information.₅₀The specific activity of human IL-20, as determined by a cell proliferation assay using co-transfected murine BaF3 pro-B cells with human IL-20Rα and human IL-20Rβ, is greater than 6.7 × 10, and the corresponding concentration is less than 1.5 ng/ml. These results indicate that human IL-20 is highly potent and capable of stimulating cellular proliferation at very low concentrations. These findings have important implications for the design of future therapeutic interventions targeting IL-20-dependent pathways. Overall, this study provides valuable insights into the biological activity of human IL-20 and highlights its potential as a promising therapeutic target for a range of diseases.⁵ IU/mg.

Reconstitution

It is recommended to briefly centrifuge the vial before opening to ensure the contents have settled to the bottom. Reconstitution should be done with 100 mM HAC to achieve a concentration of 0.1-1.0 mg/mL. For long-term storage, it is advised to aliquot the stock solution and store at ≤ -20 °C. Any further dilutions should be carried out using appropriate buffered solutions. It is important to follow these steps to ensure the best results and maintain the integrity of the product.

Stability & Storage

To ensure the stability of this lyophilized preparation, it is recommended to store it at 2-8 °C. However, for long-term storage, it is best to keep it at -20 °C in a desiccated state. Once reconstituted, the preparation can last up to one week in the refrigerator at 2-8 °C. For optimal stability, divide the reconstituted preparation into working aliquots and store them at a temperature between -20 °C to -70 °C. It is advisable to avoid subjecting the preparation to repeated freeze/thaw cycles as it may compromise its quality.

Target

Accession

Q9UHD0

Gene IDs

29949

References

References

1. Chang C, Magracheva E, Kozlov S, et al. 2003. J Biol Chem, 278: 3308-13.

2. Gallagher G, Dickensheets H, Eskdale J, et al. 2000. Genes Immun, 1: 442-50.

3. Liao YC, Liang WG, Chen FW, et al. 2002. J Immunol, 169: 4288-97.