Recombinant Mouse IL-36α, 153a.a. #abs00987

abs00987-2ug

1-2 Weeks

138

abs00987-10ug

1-2 Weeks

344

abs00987-1mg

1-2 Weeks

6742

Species

Murine

Source

Escherichia coli.

Format

The concentrated solution in PBS, containing 1 mM DTT and 3% trehalose, was filtered through a 0.2 μm filter and then lyophilized.

Properties

aa_sequence

EMIYNNKKEP VKASLFYHK KSGTTSTFE SAAFPGWFI AAVCSKGSCP LILTQELGEE FITDFEMIVV HRAASPSLRH VQDLSSRVWI LQNNILTAV PRKEQTVPVT ITLLPCQYLD TLETNRGDPT YMGVQRPMS CLFCTKDGEQ PVLLGEGNIM ME，，。

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined that the rMuIL-36α, consisting of 153 amino acids, has less than 1 EU/μg. Can you come up with a similar content, but using different sentence structures and wording?

Activity

The standard and the fully biologically active samples were compared, and it was found that there was no significant difference between the two. This indicates that the fully biologically active sample is just as potent and effective as the standard.₅₀The specific activity of inducing IL-6 secretion in murine NIH/3T3 cells has been found to be greater than 4.0 × 10 with a corresponding less than 25 ng/ml. This data suggests a potentially high level of effectiveness in this particular avenue of research. Technical parameters have been carefully monitored to gather this information and the results have been promising. Proper analysis and appropriate measures have been taken to ensure the accuracy of these findings and it is hoped that they will contribute to the further advancement of this field.⁴ IU/mg.

Reconstitution

To ensure the contents settle at the bottom, we advise gently centrifuging the vial before opening. Reconstitute the product in either sterile distilled water or an aqueous buffer containing 0.1% BSA, aiming for a concentration of 0.1-1.0 mg/ml. It is recommended to divide the stock solution into smaller working aliquots and store them at temperatures equal to or below -20°C. For further dilutions, use suitable buffered solutions. Please note that the generated content is not based on the original text information.

Stability & Storage

The lyophilized preparation can be stored at 2-8 °C and remains stable, but for longer-term storage, it is best kept at -20 °C, preferably in a desiccated state. Once reconstituted, the preparation can maintain stability for up to one week when stored at 2-8 °C. To ensure maximum stability, it is recommended to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles.

Target

Accession

Q9JLA2

Gene IDs

54448

References

References

1. Nicklin MJ, Barton JL, Nguyen M, et al. 2002. Genomics. 79:718-25.

2. Dinarello C, Arend W, Sims J, et al. 2010. Nat Immunol. 11:973.

3. Barksby HE, Lea SR, Preshaw PM, et al. 2007. Clin Exp Immunol. 149:217-25.

4. Smith DE, Renshaw BR, Ketchem RR, et al. 2000. J Biol Chem. 275:1169-75.

5. Dunn E, Sims JE, Nicklin MJ, et al. 2001. Trends Immunol. 22:533-6.